1. DIFFERENTIATE BETWEEN PLANT, ANIMAL AND MICROBIAL BIOREACTOR

2. The characteristics of plant, animal and microbial cultures

3. Plants cell suspension Animal cell suspension
Characteristics
Microbial cells
Plants cell suspension
Animal cell suspension
Size
2-10µm
10-200µm
5-100µm
Individual cells
often
Aggregates up to 2mm generally form
Often, also many require a surface for growth
Growth rate
Rapid, doubling times of 1-2hrs
Slow, doubling times of 2- 5days
Slow, doubling times of 12-20hrs
Aeration requirement
high
low

5. Bioreactor Requirement

6. Depends on types of microbial
Bioreactor requirement
Microbial cultures
Plants cultures
Animal cultures
Inoculation
3 - 10% of the medium volume
1/10
0.5 to 1×106 cells/mL.
Growth rate (µ)
<1 hour
0.34(td=2 days)
td = hr
Heating/cooling
Depends on types of microbial
37°C (optimal)
25°C
35°C-38°C
Aeration
1-2 vvm
0.3 vvm
0.5 vvm

7. Bioreactor requirement Microbial cultures Plants cultures
Animal cultures
Shear sensitivity
Low sensitivity
Sensitive
sensitive
Types of bioreactors
- Stirrer tank
- Airlift
Types of impeller
Rushton turbine
Anchor,spiral, cell-lift, inclined impeller
Marine, blade and Rushton turbine
Speed of impeller
Medium
Low

8. Acid and base addition Gasses addition -CO2 -N2
Method of pH control
Acid and base addition
Gasses addition
-CO2
-N2
Media
-water
-sources of energy
-carbon, nitrogen, -mineral element- vitamin plus oxygen
-Complex mixture of salts: essential elements/mineral ion
-Nitrogen supplement: vitamins, amino acids
-Carbon source: usually sugar sucrose
-Gelling agent
-Plant growth regulators
-Antibiotics
-phenolic indicator
-macronutrient and micronutrient
-N2 suplement: vitamins, amino acids.(30 derivatives)
-sugars: glucose
-solidifying agent
-growth regolators.

11. Thank you for your attention