1. Zeiss Neon 40 EsB Sample Insertion/Removal
Close the column chamber valve and make sure sample stage is at the exchange position
Click on ‘Close Column Chamber Valve’ under the Airlock panel
Using the ChamberScope, make sure sample stage is at 0o tilt and lowered out of the way of the SEM/FIB columns
Under the Stage Points List panel, double click on $exchange to drive the stage to the exchange position

2. Zeiss Neon 40 EsB Sample Insertion/Removal
Load samples (or not if removing) and evacuate the airlock
While wearing gloves pick an appropriate stage and load the samples
Open the airlock door (swivel the monitor out of the way) and fit the dovetail stage securely onto one of the two teflon holders
On the airlock, the red ‘Wait’ light should be lit as well as the yellow ‘Rod Status’ and ‘Gate Closed’ lights
While holding the chamber door closed, push the ‘Pump’ button (green light on) followed by the ‘Open’ button (green light on)
When the green ‘Proceed’ light turns on and the yellow ‘Gate Closed’ light turns off, wait until the gate valve fully opens (~30 seconds, look for motor rolling to cease) and the sample chamber lights up

3. Zeiss Neon 40 EsB Sample Insertion/Removal
Insert or remove the specimen stage from the SEM chamber
If necessary, line up the sample insertion rod with the position of the sample using the knob underneath the rod
Release the lock on the sample insertion rod and carefully screw the sample insertion rod into the stage
Be careful not to push the stage off the holder and do not over tighten the rod
Insert the sample into the SEM chamber fitting the dovetail stage onto the holder securely
Unscrew the sample insertion rod from the sample holder (Rod Status light should be solid yellow, not blinking otherwise system will not proceed to the next step), retract it all the way and lock it into the fully withdrawn position

4. Zeiss Neon 40 EsB Sample Insertion/Removal
Vent the airlock to atmosphere and open the column chamber valve
Release the ‘Open’ button (green light off) and push the ‘Close’ button (green light on) to close the gate valve
Wait until the ‘Gate Closed’ yellow light comes on
Release the ‘Pump’ button (green light off) and then the ‘Close’ button (green light off)
Push the ‘Purge’ button (green light on) to vent the airlock to atmosphere
The red ‘Wait’ light will turn on
When the airlock is fully vented (~ 10 seconds), release the ‘Purge’ button (green light off)
Open the column chamber valve on the Airlock panel