1. EVALUATION OF ANTI-PARKINSON ACTIVITY OF LEMON GRASS Miss.Rupali Dilip Handal T. Y B. Pharmacy GUIDED BY Prof- Hemant. J. Pagar (M. Pharm. Pharmacology)

2. CONTENT Introduction Objectives Review of literature Methodology Experimentation Result Conclusion References

3. INTRODUCTION Parkinsons disease is a Neurodegenerative disorder of central nervous system.It was first described in 1817 by James Parkinson. This disease is occure due to low level of dopamine. SYMPTOMS OF DISEASE  Tremor  Rigidity  Bradykinesia  Depression

4. OBJECTIVES To study the Anti-Parkinson Activity of cymbopogon citratus (lemon grass). The lemon grass plant having the anti- parkinson activity, The present study is planed with the following objectives To prepare the ethanolic extract of lemon grass. To evaluate the traditional claim of its anti-parkinson activity. To determime probable action of the extract under study of its anti-parkinsons activity.

5. REVIEW OF LITERATURE Name of plant- cymbopogon citrates Synonym- lemon grass, gavati chaha Family- poaceae

6. METHODOLOGY  Plant material  Animals  Determination of acute toxicity study (LD 50 ) IN-VIVO STUDIES  Haloperidol-Induced catalepsy in rats Neuroleptic agent also induced catalepsy, but with different mechanism. Neuroleptics inhibit dopamine D 2 receptar in the substantia nigra. Since the catalepsy is a common extrapyramidal side effect of neuroleptic agents. This experiment carried out by using block method.

7. EXPERIMENTATION Sr noAnimal for testingDrugBody wtDose 1NormalDistilled water2150.1 ml 2Negative controlHaloperidol1700.15 ml 3Positive control LDopa+ haloperidol 162 20.25mg+0.16 ml 4Test 1 Test drug + haloperidol 200200mg+0.2ml 5Test 2 Test drug + haloperidol 150400mg+0.15 6Test 3Test drug + haloperidol 180800mg+0.18ml

8. Normal00 0 ± 0 Negative control (Haloperidol) ## 012.53.5 2.8 ± 0.48 Positive control (levodopa carbidopa + haloperidol) ** 000.501 050.6± 0.18 Test 1 C.C Extract 200mg 002.5 0.51.8 ± 0.48 Test C.C Extract 400mg** 001.5 0.51 ± 0.31 Test C.C Extract 800mg** 00011.5010.50.8 ± 0.25 Treatment (Dose in mg/kg) Duration of Catelepsy in min at Effect of ethanolic extract of lemon grass RESULT

9. Fig- Animal showing 3 rd stage of catatonia

10. Fig-Animal showing 4 th stage of catatonia

11. CONCLUSION The chymbopogon citratus extract 400 mg and 800 mg shows significant effect when compared with that of standard. So, the test extract exibit anti-parkinson activity, which may be due to the phytochemical constituents present in the extract of chymbopogon citratus. The lemon grass contains a chemical constituent i.e. Citral which has a close structural similarity with that of dopamine. So, our activity may be due to this structural similarity to dopamime. Hence, further studies need to be carried out in this regard to verify the activity of lemon grass.

12. REFERENCES  Kokate CK, Purohit AP, Gokhle SB. Pharmacognosy,13 th edition. Nirali Prakashan: pune,India, 1994  Kulkarni SK. Handbook of Experimental Pharmacology,3 rd edition. Vallabh Prakashan: Delhi, India,2004  Essentials of Medical Pharmacology six edition.KD tripathi page no 414- 422.  Rang and Dales Pharmacology Seventh edition page no.485-489,463.  Lippincott’s illustrated reviews Pharmacology 4 th edition page no.94-100.