1. ?

2. gfp-gene as cDNA in a host-DNA-fragment E. coli (new host)  gfp ? ??????? Aequorea victoria (donor) host

3. gfp-gene as cDNA in a host-DNA-fragment DNA-transport system E.coli (new host)  gfp host

4. gfp-gene as cDNA in a host-DNA-fragment DNA-transport system E.coli (new host)  13 cleavage of the interesting gene with appr. restriction enzymes gfp purified cloning vector DNA preparation of DNA-incor- porating cells gfp 4 host

5. gfp-gene as cDNA in a host-DNA-fragment DNA-transport system E. coli (new host)  13 cleavage of the interesting gene with appr. restriction enzymes gfp purified cloning vector DNA preparation of DNA-incor- porating cells gfp??? 4 ? ? identification/verification??? host

6. gfp-gene as cDNA in a host-DNA-fragment DNA-transport system E. coli (new host)  13 cleavage of the interesting gene with appr. restriction enzymes gfp purified cloning vector DNA preparation of DNA-incor- porating cells (competent cells) gfp 4 2 joining of vector DNA and gene fragment (ligation) cleavage of vector DNA importation of constructed DNA (transformation ) cultivation of cells 3 electrophoresis recovery of gfp-fragment from agarose gel gfp ? host 4