1. Biomanufacturing At Mount Wachusett Community College Funded in full or in part by the U.S. Department of Labor Grant CB-17329-08-60-A-25

2. Associate’s Degree Funded in full or in part by the U.S. Department of Labor Grant CB-17329-08-60-A-25 Number Suggested Course Order Cr BIO109 General Biology 4 BTC101 Introduction to Biotechnology 4 BTC102 Basic Solution & Media Preparation 3 ENG101 English Composition I 3 General Elective 3 CHE203 Introduction to Inorganic, Organic and Biochemistry 4 Professional Elective 3/4 CIS127 Computer Technologies 3 ENG102 English Composition II 3 MAT143 Probability and Statistics 3 BIO160 Principles of Biochemistry 4 BTC110 Biotechnology Experience: Concepts and Procedures 4 HUM212 Medical Ethics 3 PER126/130 Health, Fitness, & Wellness Elective 2/3 BTC120 Biotechnology Experience: Biomanufacturing 4 BTC200 Biotechnology Seminar 3 BIO170 Cell Biology 4 Social Science Elective 3 Total: 60/62 credits

3. Class Format 4 credit class  Two lectures/week – 75 minutes each  One lab/week – 2 hours ◦OR  One class/lab per week – 4 ½ hours Workforce Training Program ◦3 days/week – 7 hours/day Funded in full or in part by the U.S. Department of Labor Grant CB-17329-08-60-A-25

4. BTC120 Biomanufacturing Course Schedule Funded in full or in part by the U.S. Department of Labor Grant CB-17329-08-60-A-25

5. Workforce Training Program Funded in full or in part by the U.S. Department of Labor Grant CB-17329-08-60-A-25

6. Aseptic Technique Very important! Funded in full or in part by the U.S. Department of Labor Grant CB-17329-08-60-A-25

7. Upstream Processing CHO cells ◦Contain gene for tPA Approximately 3 weeks ◦100ml spinner flasks ◦3L bioreactors Funded in full or in part by the U.S. Department of Labor Grant CB-17329-08-60-A-25

8. Downstream Processing Centrifugation Low pressure liquid chromatography ◦Ion exchange to purify tPA ELISA SDS-PAGE Funded in full or in part by the U.S. Department of Labor Grant CB-17329-08-60-A-25