1. Online Resource Role of endothelial Nox2 NADPH oxidase in angiotensin II-induced hypertension and vasomotor dysfunction. Colin E Murdoch, Sara P Alom-Ruiz, Minshu Wang, Min Zhang, Simon Walker, Bin Yu, Alison Brewer, Ajay M Shah Basic Research in Cardiology King’s College London British Heart Foundation Centre, Cardiovascular Division, London SE5 9PJ, UK. Corresponding author ajay.shah@kcl.ac.uk

2. Online Resource 1: Comparison of body and organ weight from Nox2Tg and WT littermate controls.

3. Nox2 Actin Online Resource 2: (A) Nox2 protein levels in aorta from line 2 Nox2 Tg mice (*= P 3). * eNOS actin WT Tg C B A

4. Online Resource 3: Effect of in vivo angiotensin II treatment on aortic antioxidant mRNA levels. WT and Tg groups were treated with either saline or AngII (1.1mg/kg/day). There were no differences between Wt and Tg in the response to AngII by 2-way ANOVA. Asterisks indicate significant effect (p 3 per group.