1. How to Screen out the High Productive Algae? Qian Zhou 10/16/2009

2. What is the criteria? Daily biomass? Nile Red Staining? Biomass carbon and nitrogen percentage? Daily lipids? Daily carbon? Daily reduced carbon? (Batch)Total biomass in a relatively shot period? (Batch)Total lipids in a relatively shot period? Photosynthetic efficiency? Nitrogen effects?

3. Figure1: daily biomass production of 12 algae stains What we have done?

4. Method of lipid determination Gas Chromatography Nile Red Staining TD-NMR/ Regular NMR

5. TD-NMR Time domain NMR is an alternative method for lipid analysis. Highly accurate, no pretreatment needed Could determine the lipid concentration in real- time

6. basic principles of td-NMR

7. Plans for next step Determine the daily total biomass and lipids of 6~8 species out of 30 strains Determine the Phi Ⅱ of the 6 species Determine the carbon dioxide utilization of these species Determine the nitrogen utilization of these species Compare FAME analysis with NMR data

8. Thank you!