1. www.jst.umn.edu Chromium Reagents H 2 CrO 4, CrO 3, PCC, PDC, CrO 2 Cl 2 Finding alternatives is highly encouraged!

2. www.jst.umn.edu Hazards of Cr Reagents Inhalation => breathing difficulties, gastrointestinal and neurological effects. Dermal exposure => skin burns. Cr(VI) is more toxic than Cr(III). Cr(VI) is carcinogenic => lung cancer upon inhalation 3 – 4 mg could be fatal!!! 2 Clinical Toxicology Jan 1999, Vol. 37, No. 2: 173–194 Read More: http://informahealthcare.com/toc/ctx/37/2http://informahealthcare.com/toc/ctx/37/2

3. www.jst.umn.edu Handling Cr compounds Use Cr compounds only after proper training and approval by your PI. Use only in fume hood. Use cotton lab coat, gloves, face mask and goggles. Work with low hood sash. Store separately from oxidizers, strong acids or bases. 3

4. www.jst.umn.edu Spills, Waste & Clean-up Have a separate, designated Cr waste bottle(s). – Dispose contaminated gloves/clothing/paper towels in appropriate Cr waste. Contain spills inside hood with sand/vermiculite. Wipe up and dispose in designated waste bottle. For large spills outside hood, vacate lab and contact DEHS. Make sure to remove Cr residues from glassware before cleaning them. 4