1. Kristen Jevsevar Jason McGill Sean Mercado Rebecca Tarrant

2. Problem Statement  Cells consume different amounts of glucose under different environmental situations  Problem: How to quantify glucose or other metabolite consumption/production on a scale of microliters?

3. Performance Criteria  Needs to work for at least 24 hours  Should recalibrate automatically to account for electrochemical drift  Must be able to measure glucose concentrations between 0mM and 6mM

4. Primary Objective  Design a glucose electrode interface that will measure micro-scale concentrations while maintaining affordability

5. Solution Description  Pine Instruments makes a ceramic platinum screen printed electrode that we will be interfacing to a microfluidic system

6. Goals  Interface a microfluidic channel with our ceramic electrode  Create a pumping system which can self-calibrate  Make it more affordable

7. Factors  Smaller volumes yield more accurate results  PDMS and ceramic electrodes are inexpensive

8. Performance Metrics  Calibration curves Linear correlation Measurable range of concentrations  Flow capacity  Control tests with known concentrations

9. Experimental Setup  A clamp is used to seal the PDMS to the ceramic electrode  Solution containing glucose is run through the channel and measured

10. Previous Work  A clamp was designed for proper sealing  Rough PDMS channels were made to fit the ceramic electrode  Calibration curves of the electrode simply in a beaker containing differing amounts of glucose have been made

11. Current Work  Designing a more reproducible channel using a CNC end mill  Setting up a calibration experiment for stop/flow and continuous pumping

12. Future Work  Testing the sealing characteristics of the new design  Testing the system while using cell media  Designing a pumping system for the PDMS/ceramic electrode component