1. WORLD BIO SUMMIT & EXPO November 02 – 04, 2015 Dubai, UAE TITLE : TREATMENT OF TYPE 2 DIABETES AND ITS COMPLICATIONS WITH AN INNOVATIVE BIOTECHNOLOGY BASED ON ZEBRAFISH EMBRYOS STEM CELLS DIFFERENTIATION STAGE FACTORS SPEAKER : GUIDO NORBIATO Department of Special Diseases, Endocrinology and Metabolism University Hospital L. Sacco, Milan (Italy) email : guidonorbiato@libero.it Slide 1

2. Slide 2 LET’S LOOK NOW AT THE MAIN MECHANISMS BY WHICH STRESS AND NON RESOLVED INFLAMMATION ATTACK DIABETES PATIENTS First : - Damage to stem cells Second :- Imbalance and/or the destruction of the adaptive systems Third :- Impairment of the immune system function, affecting immune cell turnover, replication, exhaustion and longevity Fourth :- Deregulation of nuclear steroid receptors Fifth :- Induction of epigenetic alterations, such as aberrant DNA methylation and erosion, and shortening of telomeres which is associated with a heightened risk of neuro-endocrine-immune- metabolic disorders and premature aging.

3. Slide 3 EXPOSURE TO SCDSFs ENHANCES STEM CELLS EXPRESSION AND MULTIPOTENCY ENHANCES IMMUNE-MODULATORY AND PROTECTIVE EFFECTS OF MESENCHYMAL STEM CELLS ACTIVATES TELOMERES IN NORMAL AND MULTIPOTENT STEM CELLS PROLONGS STEM CELLS LIFE BY EXTENDING TELOMERES REVERSES EPIGENETIC ALTERATIONS BY INHIBITING DNA METHYLTRANSFERASES AND HISTONE ACETILASES IMMUNE CELLS LONGEVITY INCREASES IMMUNE CELLS LONGEVITY WHICH IS DIRECTLY CONNECTED TO THE FUNCTIONAL INTEGRITY OF TELOMERES INDUCES DIFFERENTIATION, SLOWS DOWN PROLIFERATION AND INCREASES APOPTOSIS OF CANCER (STEM) CELLS INCREASES DIFFERENTIATION AND/OR APOPTOSIS OF DEGENERATED NEURAL CELLS CONTRASTS NEURO-DEGENERATION