1. B.E Pruitt & Jane J. Stein Chapter 9, part B Biotechnology and Recombinant DNA

2. Genetic Engineering Figure 9.11.1

3. Genetic Engineering Figure 9.11.2

4. Making a Gene Product Figure 9.12.1

5. Making a Gene Product Figure 9.12.2

6. Used because it is easily grown and its genomics are known Need to eliminate endotoxin from products Cells must be lysed to get product E. coli

7. Making a Gene Product Figure 9.13

8. Subunit vaccines Nonpathogenic viruses carrying genes for pathogen's antigens as vaccines Gene therapy to replace defective or missing genes Human Genome Project Nucleotides have been sequenced Human Proteome Project may provide diagnostics and treatments Therapeutic Applications

9. Random Shotgun Sequencing Figure 9.14

10. Understanding of DNA Sequencing organisms' genomes DNA fingerprinting for identification Scientific Applications Figure 9.16

11. Southern Blotting Figure 9.15.1

12. Southern Blotting Figure 9.15.2

13. Southern Blotting Figure 9.15.3

14. Agricultural Applications Table 9.2

15. Genetic Engineering Using Agrobacterium Figure 9.18

16. Avoid accidental release Genetically modified crops must be safe for consumption and for the environment Who will have access to an individual's genetic information? Safety Issues and Ethics