1. D. KREBS CYCLE

2. 2. occurs in the matrix of the mitochondria 3. only occurs if oxygen is present 1. Krebs cycle allows the cell to get more energy out of the acetyl-CoA product of the oxidation of pyruvate

3. remember the cell is trying to release NAD + to allow glycolysis to continue produce ATP

4. COOH O=C CH 2 COOH CH 2 HOC – COOH CH 2 COOH acetyl-CoA CH 3 C=O S-CoA 4. Details H2OH2O CoA-SH hydrase oxaloacetate citrate

5. COOH CH 2 HOC – COOH CH 2 COOH CH 2 HC – COOH HOCH COOH isomerase citrate isocitrate

6. COOH CH 2 HC – COOH HOCH COOH CH 2 C=0 COOH NAD + NADH + H + CO 2 dehydrogenase decarboxylase isocitrate alpha-ketoglutarate

7. COOH CH 2 C=0 COOH CH 2 C=O S – CoA NAD + NADH + H + CO 2 CoASH dehydrogenase decarboxylase alpha-ketoglutarate succinyl-CoA

8. COOH CH 2 C=O S – CoA COOH CH 2 COOH CoASH GTP GDP + Pi ADP ATP phosphorylase H2OH2O hydrase succinyl-CoA succinate

9. COOH CH 2 COOH CH ll HC COOH FAD + H2H2 dehydrogenase FAD succinate fumarate

10. COOH CH ll HC COOH HOCH CH 2 COOH H2OH2O hydrase fumarate malate

11. COOH HOCH CH 2 COOH O=C CH 2 COOH NAD + NADH + H + dehydrogenase malate oxaloacetate

12. START AGAIN COOH O=C CH 2 COOH CH 2 HOC – COOH CH 2 COOH acetyl-CoA CH 3 C=O S-CoA Oxaloacetate citric acid so this is sometimes called the citric acid cycle

13. 5. EQUATION: acetyl-CoA +3 NAD + +FAD + +H 2 0 +ADP + Pi 2 CO 2 +3 [NADH + H + ] +FADH 2 +ATPCoASH +

14. E. SUMMARY – so far for HIGH ENERGY PRODUCTS where glucoseglycolysis cytoplasm 2 ATP oxidation of pyruvate matrix of mitochondria 2 [NADH + H + ] 4 ATP

15. oxidation of pyruvate Kreb’s cycle 2 [NADH + H + ] 2 ATP 6 [NADH + H + ] 2 FADH 2 matrix of mitochondria where

17. remember the cell is trying to release NAD + to allow glycolysis to continue produce ATP Krebs made no NAD +, it used 6 Krebs made only 2 more ATP