1. January 2004 SundayMondayTuesdayWednesdayThursdayFridaySaturday 123 45 Mice arrive□ Mite spray □ Med feed Mice arrive □ Normal feed Mice arrive910 1112 Mice arrive Contacts arrive □ Group house contacts □ Mite spray □ Normal feed □ Mice arrive □ Mite spray □ Med feed □ Add/assign contacts 151617 18 Contact testing 20□ Mite spray □ Normal feed □ Mite spray □ Normal feed □ Move contacts 22 Results expected 24 25 Mice released27□ Mite spray □ Med feed □ Separate contacts 293031 Group 1-January (to arrive 1/6-1/14) Mite spray= treat all mice (see SOP) with mite spray weekly Med feed= use fenbendazole-medicated feed (usually weeks 2,4 and 6) Contacts= contact sentinel mice; moved to new cage once weekly, first 2 weeks, then separated into individual cages and used as a standard (bedding) sentinel Testing= diagnostic lab testing (serology, parasitology) Results= serology results (from Charles River Laboratories) Release= mice released from quarantine; mice ready for transfer to colony See Mouse Quarantine-Tech SOP for more detailed information on standard operating procedures for mouse quarantine

2. February 2004 SundayMondayTuesdayWednesdayThursdayFridaySaturday 1 2 Mice arrive□ Mite spray □ Normal feed Mice arrive □ Normal feed Mice arrive67 89 Contacts arrive □ Group house contacts □ Mite spray □ Med feed Mice arrive □ Normal feed □ Add/assign contacts Mice arrive1314 151617□ Mite spray □ Normal feed □ Mite spray □ Med feed □ Move contacts 192021 22 Contact testing 24□ Mite spray □ Normal feed □ Mite spray □ Normal feed □ Separate contacts 26 Results expected Mice released 28 29 Group 2-February (to arrive 2/3-2/12) Mite spray= treat all mice (see SOP) with mite spray weekly Med feed= use fenbendazole-medicated feed (usually weeks 2,4 and 6) Contacts= contact sentinel mice; moved to new cage once weekly, first 2 weeks, then separated into individual cages and used as a standard (bedding) sentinel Testing= diagnostic lab testing (serology, parasitology) Results= serology results (from Charles River Laboratories) Release= mice released from quarantine; mice ready for transfer to colony See Mouse Quarantine-Tech SOP for more detailed information on standard operating procedures for mouse quarantine

3. March 2004 SundayMondayTuesdayWednesdayThursdayFridaySaturday 1 Mice arrive□ Mite spray □ Med feed Mice arrive □ Normal feed Mice arrive56 78 Contacts arrive □ Group house contacts □ Mite spray □ Normal feed Mice arrive □ Normal feed □ Add/assign contacts Mice arrive1213 141516□ Mite spray □ Med feed □ Mite spray □ Med feed □ Move contacts 181920 21 Contact testing 23□ Mite spray □ Normal feed □ Mite spray □ Normal feed □ Separate contacts 25 Results expected 27 28 Mice released30□ Mite spray □ Med feed Group 3-March (to arrive 3/2-3/11) Mite spray= treat all mice (see SOP) with mite spray weekly Med feed= use fenbendazole-medicated feed (usually weeks 2,4 and 6) Contacts= contact sentinel mice; moved to new cage once weekly, first 2 weeks, then separated into individual cages and used as a standard (bedding) sentinel Testing= diagnostic lab testing (serology, parasitology) Results= serology results (from Charles River Laboratories) Release= mice released from quarantine; mice ready for transfer to colony See Mouse Quarantine-Tech SOP for more detailed information on standard operating procedures for mouse quarantine

4. April 2004 SundayMondayTuesdayWednesdayThursdayFridaySaturday 123 45 Mice arrive□ Mite spray □ Normal feed Mice arrive □ Normal feed Mice arrive910 1112 Mice arrive Contacts arrive □ Group house contacts □ Mite spray □ Med feed Mice arrive □ Normal feed □ Add/assign contacts Mice arrive1617 18 Contact testing 20□ Mite spray □ Normal feed □ Mite spray □ Med feed □ Move contacts 22 Results expected 24 25 Mice released27□ Mite spray □ Normal feed □ Separate contacts 2930 Group 4-April (to arrive 4/6-4/15) Mite spray= treat all mice (see SOP) with mite spray weekly Med feed= use fenbendazole-medicated feed (usually weeks 2,4 and 6) Contacts= contact sentinel mice; moved to new cage once weekly, first 2 weeks, then separated into individual cages and used as a standard (bedding) sentinel Testing= diagnostic lab testing (serology, parasitology) Results= serology results (from Charles River Laboratories) Release= mice released from quarantine; mice ready for transfer to colony See Mouse Quarantine-Tech SOP for more detailed information on standard operating procedures for mouse quarantine

5. May 2004 SundayMondayTuesdayWednesdayThursdayFridaySaturday 1 23 Mice arrive□ Mite spray □ Med feed Mice arrive □ Normal feed Mice arrive78 910 Mice arrive Contacts arrive □ Group house contacts □ Mite spray □ Normal feed Mice arrive □ Normal feed □ Add/assign contacts Mice arrive1415 16 Contact testing 18□ Mite spray □ Med feed □ Mite spray □ Med feed □ Move contacts 20 Results expected 22 23 Mice released25□ Mite spray □ Normal feed □ Separate contacts 272829 3031 Group 5-May (to arrive 5/4-5/13) Mite spray= treat all mice (see SOP) with mite spray weekly Med feed= use fenbendazole-medicated feed (usually weeks 2,4 and 6) Contacts= contact sentinel mice; moved to new cage once weekly, first 2 weeks, then separated into individual cages and used as a standard (bedding) sentinel Testing= diagnostic lab testing (serology, parasitology) Results= serology results (from Charles River Laboratories) Release= mice released from quarantine; mice ready for transfer to colony See Mouse Quarantine-Tech SOP for more detailed information on standard operating procedures for mouse quarantine

6. June 2004 SundayMondayTuesdayWednesdayThursdayFridaySaturday Mice arrive□ Mite spray □ Med feed Mice arrive □ Normal feed Mice arrive45 67 Contacts arrive □ Group house contacts □ Mite spray □ Normal feed Mice arrive □ Normal feed □ Add/assign contacts Mice arrive1112 13 Contact testing 15□ Mite spray □ Med feed □ Mite spray □ Med feed □ Move contacts 17 Results expected 19 20 Mice released22□ Mite spray □ Normal feed □ Separate contacts 242526 272829□ Mite spray □ Med feed Group 6-June (to arrive 6/1-6/10) Mite spray= treat all mice (see SOP) with mite spray weekly Med feed= use fenbendazole-medicated feed (usually weeks 2,4 and 6) Contacts= contact sentinel mice; moved to new cage once weekly, first 2 weeks, then separated into individual cages and used as a standard (bedding) sentinel Testing= diagnostic lab testing (serology, parasitology) Results= serology results (from Charles River Laboratories) Release= mice released from quarantine; mice ready for transfer to colony See Mouse Quarantine-Tech SOP for more detailed information on standard operating procedures for mouse quarantine

7. July 2004 SundayMondayTuesdayWednesdayThursdayFridaySaturday 123 45 Mice arrive□ Mite spray □ Normal feed Mice arrive □ Normal feed Mice arrive910 11 Contact testing Mice arrive Contacts arrive □ Group house contacts □ Mite spray □ Med feed Mice arrive □ Normal feed □ Add/assign contacts Mice arrive Results expected 17 18 Mice released20□ Mite spray □ Med feed □ Move contacts 222324 252627□ Mite spray □ Normal feed □ Separate contacts 293031 Group 7-July (to arrive 7/6-7/15) Mite spray= treat all mice (see SOP) with mite spray weekly Med feed= use fenbendazole-medicated feed (usually weeks 2,4 and 6) Contacts= contact sentinel mice; moved to new cage once weekly, first 2 weeks, then separated into individual cages and used as a standard (bedding) sentinel Testing= diagnostic lab testing (serology, parasitology) Results= serology results (from Charles River Laboratories) Release= mice released from quarantine; mice ready for transfer to colony See Mouse Quarantine-Tech SOP for more detailed information on standard operating procedures for mouse quarantine

8. August 2004 SundayMondayTuesdayWednesdayThursdayFridaySaturday 12 Mice arrive□ Mite spray □ Med feed Mice arrive □ Normal feed Mice arrive67 89 Contacts arrive □ Group house contacts □ Mite spray □ Normal feed Mice arrive □ Normal feed □ Add/assign contacts Mice arrive1314 15 Contact testing 17□ Mite spray □ Med feed □ Mite spray □ Med feed □ Move contacts 19 Results expected 21 22 Mice released24□ Mite spray □ Normal feed □ Separate contacts 262728 293031 Group 8-August (to arrive 8/3-8/12) Mite spray= treat all mice (see SOP) with mite spray weekly Med feed= use fenbendazole-medicated feed (usually weeks 2,4 and 6) Contacts= contact sentinel mice; moved to new cage once weekly, first 2 weeks, then separated into individual cages and used as a standard (bedding) sentinel Testing= diagnostic lab testing (serology, parasitology) Results= serology results (from Charles River Laboratories) Release= mice released from quarantine; mice ready for transfer to colony See Mouse Quarantine-Tech SOP for more detailed information on standard operating procedures for mouse quarantine

9. September 2004 SundayMondayTuesdayWednesdayThursdayFridaySaturday □ Mite spray □ Med feed 234 56 Mice arrive□ Mite spray □ Normal feed Mice arrive □ Normal feed Mice arrive1011 12 Contact testing Mice arrive Contacts arrive □ Group house contacts □ Mite spray □ Med feed Mice arrive □ Normal feed □ Add/assign contacts Mice arrive Results expected 18 19 Mice released21□ Mite spray □ Med feed □ Move contacts 232425 262728□ Mite spray □ Normal feed □ Separate contacts 30 Group 9-September (to arrive 9/7-9/16) Mite spray= treat all mice (see SOP) with mite spray weekly Med feed= use fenbendazole-medicated feed (usually weeks 2,4 and 6) Contacts= contact sentinel mice; moved to new cage once weekly, first 2 weeks, then separated into individual cages and used as a standard (bedding) sentinel Testing= diagnostic lab testing (serology, parasitology) Results= serology results (from Charles River Laboratories) Release= mice released from quarantine; mice ready for transfer to colony See Mouse Quarantine-Tech SOP for more detailed information on standard operating procedures for mouse quarantine

10. October 2004 SundayMondayTuesdayWednesdayThursdayFridaySaturday 12 34 Mice arrive□ Mite spray □ Med feed Mice arrive □ Normal feed Mice arrive89 1011 Mice arrive Contacts arrive □ Group house contacts □ Mite spray □ Normal feed Mice arrive □ Normal feed □ Add/assign contacts Mice arrive1516 17 Contact testing 19□ Mite spray □ Med feed □ Mite spray □ Med feed □ Move contacts 21 Results expected 23 24 Mice released26□ Mite spray □ Normal feed □ Separate contacts 282930 31 Group 10-October (to arrive 10/5-10/14) Mite spray= treat all mice (see SOP) with mite spray weekly Med feed= use fenbendazole-medicated feed (usually weeks 2,4 and 6) Contacts= contact sentinel mice; moved to new cage once weekly, first 2 weeks, then separated into individual cages and used as a standard (bedding) sentinel Testing= diagnostic lab testing (serology, parasitology) Results= serology results (from Charles River Laboratories) Release= mice released from quarantine; mice ready for transfer to colony See Mouse Quarantine-Tech SOP for more detailed information on standard operating procedures for mouse quarantine

11. November 2004 SundayMondayTuesdayWednesdayThursdayFridaySaturday 1 Mice arrive□ Mite spray □ Med feed Mice arrive □ Normal feed Mice arrive56 78 Contacts arrive □ Group house contacts □ Mite spray □ Normal feed Mice arrive □ Normal feed □ Add/assign contacts Mice arrive1213 14 Contact testing 16□ Mite spray □ Med feed □ Mite spray □ Med feed □ Move contacts 18 Results expected 20 21 Mice released23□ Mite spray □ Normal feed □ Separate contacts 252627 282930 Group 11-November (to arrive 11/2-11/11) Mite spray= treat all mice (see SOP) with mite spray weekly Med feed= use fenbendazole-medicated feed (usually weeks 2,4 and 6) Contacts= contact sentinel mice; moved to new cage once weekly, first 2 weeks, then separated into individual cages and used as a standard (bedding) sentinel Testing= diagnostic lab testing (serology, parasitology) Results= serology results (from Charles River Laboratories) Release= mice released from quarantine; mice ready for transfer to colony See Mouse Quarantine-Tech SOP for more detailed information on standard operating procedures for mouse quarantine

12. December 2004 SundayMondayTuesdayWednesdayThursdayFridaySaturday □ Mite spray □ Med feed 234 56 Mice arrive□ Mite spray □ Normal feed Mice arrive □ Normal feed Mice arrive1011 12 Contact testing Mice arrive Contacts arrive □ Group house contacts □ Mite spray □ Med feed Mice arrive □ Normal feed □ Add/assign contacts Mice arrive Results expected 18 19 Mice released21□ Mite spray □ Med feed □ Move contacts 232425 262728□ Mite spray □ Normal feed □ Separate contacts 3031 Group 12-December (to arrive 12/7-12/16) Mite spray= treat all mice (see SOP) with mite spray weekly Med feed= use fenbendazole-medicated feed (usually weeks 2,4 and 6) Contacts= contact sentinel mice; moved to new cage once weekly, first 2 weeks, then separated into individual cages and used as a standard (bedding) sentinel Testing= diagnostic lab testing (serology, parasitology) Results= serology results (from Charles River Laboratories) Release= mice released from quarantine; mice ready for transfer to colony See Mouse Quarantine-Tech SOP for more detailed information on standard operating procedures for mouse quarantine