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Suppl Table 1 Suppl.Tab.1. Homogeneity comparison of amino acid sequences of selective Bacterial gene products with the reserved TM1-H5-TM2 sequence of Kir6.1 NCBI Ref. Sequence No. Possible functionBacterial species Percent Identity (%) Gene name NP_669853Putative transport proteinYersinia pestis KIM10.31 NP_669263Putative transport proteinYersinia pestis KIM10.22 NP_669190Putative transport proteinYersinia pestis KIM9.63 NP_489358K + channel proteinNostoc sp PCC 71209.24 NP_695956Hypothetical membrane protein with possible transport function Bifidobacterium longum9.25 NP_416061Putative transport proteinEscherichia coli K128.66 ydfJ NP_602106Putative integral membrane transport protein Corynebacterium glutamicum 8.6 7 NP_669678Putative transport protein Yersinia pestis KIM 8 8.4 NP_298715Ion transporter Xylella fastidiosa 9a5c 9 8.4NP_629116 Putative integral membrane transport protein Streptomyces coelicolor A3(2)10 8.2NP_628015 Putative ion channel membrane protein Streptomyces coelicolor11 7.8NP_627203 Putative integral membrane transport protein Streptomyces coelicolor A3(2)12 7.4 NP_643737 Ion transporter Xanthomonas axonopodis 13 7.4NP_534284 K + -transporting ATPase A chain Agrobacterium tumefaciens str. C5814 7.4NP_416051Putative transport proteinEscherichia coli K1215ydeE 7.0NP_415044 Putative transport protein Escherichia coli K1216 ybbW 7.0NP_642608 Transport protein Xanthomonas axonopodis17 6.9NP_668944Putative ion transport proteinYersinia pestis KIM18 6.4 NP_223160 Putative K + channel protein Helicobacter pylori J99 19 6.4NP_624744Putative membrane transport proteinStreptomyces coelicolor A3(2)20 5.5NP_350262 K + -transporting ATPase B chainClostridium acetobutylicum 21 5.0NP_636095 K + -transporting ATPase B chainXanthomonas campestris 22 8.6
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Suppl Table 2 Suppl.Tab.2. Homogeneity comparison of amino acid sequences of selective bacterial gene products with the reserved TM1-H5-TM2 sequence of Kir6.2 NCBI Ref. Sequence No. Possible functionBacterial species Percent Identity (%) Gene name NP_629116Putative integral membrane transport protein Streptomyces coelicolor A3(2)10.91 NP_669190Putative transport proteinYersinia pestis KIM9.52 NP_627203Putative integral membrane transport protein Streptomyces coelicolor A3(2)9.53 NP_669263Putative transport proteinYersinia pestis KIM9.34 NP_416051Putative transport proteinEscherichia coli K129.15 NP_602106Putative integral membrane transport protein Corynebacterium glutamicum9.06 ydeE NP_628015Putative ion channel membrane protein Streptomyces coelicolor 8.8 7 NP_415044Putative transport protein Escherichia coli K12 8 8.5 NP_350262K + -transporting ATPase B chain Clostridium acetobutylicum 9 8.5 NP_624744 Putative membrane transport proteinStreptomyces coelicolor A3(2) 10 8.3NP_416061 Putative transport protein Escherichia coli K12 11 8.3NP_642608Transport protein Xanthomonas axonopodis 12 8.2 NP_298715 Ion transporter Xylella fastidiosa 9a5c 13 8.0 NP_223160 Putative K + channel protein Helicobacter pylori J99 14 7.8NP_534284K + -transporting ATPase A chainAgrobacterium tumefaciens str. C5815 7.8NP_643737 Ion transporter Xanthomonas axonopodis16 7.3NP_489358 K + channel protein Nostoc sp PCC 712017 7.2NP_669853Putative transport proteinYersinia pestis KIM18 7.2 NP_669678 Putative transport protein Yersinia pestis KIM 19 7.2NP_668944Putative ion transport proteinYersinia pestis KIM20 7.0 NP_636095 K + -transporting ATPase B chainXanthomonas campestris 21 6.3 NP_695956 Hypothetical membrane protein with possible transport function Bifidobacterium longum 22 8.9 ybbW ydfJ
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Suppl Table 3 Suppl.Tab.3. Amino acid sequence of TM1-H5-TM2 region between rvKir6.1/rvKir6.2 and the selective E. coli K12 genes Gene Name TM1 domain H5-GFG Motifs TM2 domain INAMLGCIQNIVGL ybbW ydfJ ydeE No match126INA186* --- LGL7 No match359 --- MFGC336 IVG43 No match62 IGA55 --- --- rvKir6.173-101 (28 aa)132-134 (3 aa)155-177 (22 aa) rvKir6.2 72-100 (28 aa)122-124 (3 aa)145-167 (22 aa) *amino acid sequence order
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