1. Mussie Y. Habteselassie Crop and Soil Sciences UGA Griffin Campus P & T Presentation 2013 SPRING FACULTY MEETING, MACON, GA THE SOIL MICROBIOLOGY PROGRAM

2.  70% research & 30% teaching appointment  Outline: Research Program (sample projects) Teaching Responsibilities INTRODUCTION

3. RESEARCH PROGRAM  Environmental Soil Microbiology Program (ESM) I. The Ecology Of Human And Animal Pathogens In Soils II. Environmental Services Of Soil Microbial Community III. Microbial Water Quality http://www.soilmicrobiology.griffin.uga.edu/index.html

4. I. Ecology Of Human And Animal Pathogens In Soils  Use of model microorganisms Lux/GFP marked E. coli O157:H7/Salmonella spp Antibiotic resistant E. coli/Salmonella spp Ecology of pathogens in soils Transmission to produce Survival

5. Current Projects  1. Salmonella And Norovirus Survival In Organic and Conventional Soils  2. The Role Of Microbial Diversity And Activity In The Suppression Of Salmonella Persistence In Soils  3. Soil Organic Matter Accumulation On Gloves During Lettuce Harvest and Its Effect On Pathogen Persistence: Co- PI On $338,414 Grant From UC Davis.

6. Results so far….

7. II. Environmental Services of Soil Microorganisms Soil microbes & their environmental services Pollutant remediation N & C cycling  Approach Molecular microbiology – targeting functional genes Stable isotope techniques – process characterization

8. Current Projects  4. N Cycling Under Contrasting Agricultural Management Systems  Organic vs conventional farming systems  USDA-AFRI Microbial Communities Program. Habteselassie, M.Y. (Co- PI):$499,894  Closely collaborates with Miguel Fig. Simplified Internal Soil N Cycle

9. Results so far….

10.  5. The Dynamics of Ammonia Oxidizers In Pasture Lands Under Long-term Poultry Litter Application  Eatonton and Watkinsville long term field plots  In collaboration with Miguel Cabrera Current Projects…

11.  Changes in soil N cycling organisms following repeated poultry litter application Ammonia Oxidizing Bacteria Ammonia Oxidizing Archaea Treatment Plots – Litter @ different rates Control Plots Treatment Plots – Litter @ different rates Results so far….

12.  6. Volatile Organic Compounds (VOC) Metabolizing Bacteria In Indoor Plants In collaboration with S. Pennisi and S. Kays - Horticulture Current Projects…

13. South Korea: huge interest in phytoremediation

14. Results so far….

15. III. Microbial Water Quality Emphasis Survival in contaminated water Sources Food safety link – use of contaminated water for irrigation

16.  7. Impacts of On-site Wastewater Treatment Systems (OWTS) On Water Quality and Quantity In Streams Of Urbanizing Watersheds of Metro-Atlanta Area Current Project  Supported by $567,000 NIFA Grant: M. Habteselassie, J. Clarke, D. Radcliffe, Q. Huang, M. Risse, E. Bauske, J. Mullen

17. Study Site Watershed Characteristics OWTS DensityLowHigh Drainage area (mi 2 ) 0.781.15 #OWTS/ mi 2 57559 River Basins Oconee and Ocmulgee CountyGwinnett

18. Results so far… 2012: Habteselassie, M.Y. Radcliffe, D., Clarke, J.,… Evaluation of the impact of on-site wastewater treatment systems on water quality in urbanizing watersheds. In Annual Meetings Abstracts. ASA, CSSA, and SSSA, Madison, WI. 2012: Habteselassie, M.Y. Radcliffe, D., Clarke, J.,… Watershed-scale impacts of on-site wastewater treatment systems on water quality and quantity: Results for Water Quality Indicators. In Land Grant/See Grant National Water Conference, Portland, OR. 2012: Radcliffe, D., Clarke, J., Habteselassie, M.Y.,… Watershed-scale impacts of on-site wastewater treatment systems on water quality and quantity: Results for nitrogen and base-flow yield. In Land Grant/See Grant National Water Conference, Portland, OR.

19. Results so far…

20. TEACHING #CoursesYr TaughtStudent # 1*CRSS 4610/6610: Soil MicrobiologyEvery Fall69 2CRSS 3060: Soils and HydrologyEvery Fall23 3CRSS 4580/6580: Soil Erosion and Conservation 20104 4AESC 3920: Ag. and Environmental Sc. Internship 2010, 2011 2 5AESC 4960: Undergraduate Research 2009 - 20123

21. Required Courses Course Name CRSS 4610/6610Soil Microbiology CRSS 3060Soils and Hydrology AESC 4960Undergraduate Research AESC 3510 AESC 4190/6190 CRSS 4440/6440 CRSS 3050 …TEACHING Environmental Resources Science Major @ the Griffin Campus 43%