1. The University of Manchester Faculty of Life Sciences Fundamental concepts of Drosophila genetics BIOL20332/20972 GENETICS / Dev. Biol. RSM MODULE 2 Andreas Prokop

2. Small_Fly-pt1 A brief overview of history and importance

3. Wide range of data bases and web sites A one-stop-shop for fly resources Behzad Rowshanravan

4. Gene identifiers

5. Gene location

6. Awareness of what's going on

7. Using marker mutations and balancers, concepts of lethality and stock keeping

8. Gregor Mendel (1822-1884) Mendelian rules of genetic inheritance

9. Bridges & Morgan, 1919 Thomas Hunt Morgan (1866-1945) Genetic marker mutations in fly follow the same rules

10. Keeping flies carrying genetic marker mutations e/e homozygous viable All flies are genetically identical!

11. Problems occur if mutations are homozygous lethal e/e homozygous lethal More and more non- mutant flies pop up!

12. Using genetically marked balancer chromosomes mice are individually "genotyped" and selected - but flies?!? Drosophila genetics makes use of genetically marked balancer chromosomes

13. Using genetically marked balancer chromosomes mutSer mut Ser mut / mut mut / Ser Ser / Ser

14. Using genetically marked balancer chromosomes

15. mut1Cy mut1 Ser mut1 / mut1 mut1 / Cy Cy / Cy mut2Ser mut2 Ser mut2 / mut2 mut2 / Ser Ser / Ser

16. Using modern fluorescent genetic markers mut1Cy mut1 Ser mut1 / mut1 mut1 / Cy Cy / Cy mut2Ser mut2 Ser mut2 / mut2 mut2 / Ser Ser / Ser mut1 mut2 ; 2.3. How many are: mut1/mut1; mut2/mut2 ?1/16 = 6.25%

17. Using modern fluorescent genetic markers mut1Cy mut1 Ser mut1 / mut1 mut1 / Cy Cy / Cy mut2Ser mut2 Ser mut2 / mut2 mut2 / Ser Ser / Ser

18. Using modern fluorescent genetic markers mut1Cy mut1 Ser mut1 / mut1 mut1 / Cy Cy / Cy mut2Ser mut2 Ser mut2 / mut2 mut2 / Ser Ser / Ser