1. presentation by Jennifer Unterbrink PCDU seminar November 11th, 2015
ESCRT Complex and Retromer
presentation by Jennifer Unterbrink
PCDU seminar
November 11th, 2015

3. Endosomes in plants

4. Endosomes in plants

5. Endosomes in plants heterogenous collection of organelles function:
sorting and delivery from the cell surface
transport from TGN to lysosome/ vacuole
 biosynthetic AND endocytotic pathway

6. Multivesicular bodies
Winter and Hauser, Trends in Plant Sc. Vol.11 No.3

7. Endosomal traffickingER
Golgi
secretory vesicles LE
V/ L EE PM
retrograde transport
anterograde transport
biosynthetical/ secretory transport
Bruce Alberts (Ed.), Molecular biology of the cell

8. Coats of the late endosomal system

9. Retromer schematic
SNX: phox-homology domain, allows association with phospholipids (PI3P)
Schellmann and Pimpl, 2009: Curr. Opinion in Plant Biol. 12

10. BAR domain
Bin-Amphiphysin-Rvs

11. Retromer structure

12. SNXs important for Retromer function
Rojas et al., Mol. and Cell. Biol. 02/2007

13. Plant Retromer
Schellmann and Pimpl, 2009: Curr. Opinion in Plant Biol. 12

14. ESCRTs

15. ESCRT subunits in plants
Schellmann and Pimpl, 2009: Curr. Opinion in Plant Biol. 12

16. ESCRT subunits in plants
Schellmann and Pimpl, 2009: Curr. Opinion in Plant Biol. 12

17. ESCRTs in cytokinesis
Spitzer et al., Development 133

18. The coat to perdition
2015/11/08

19. ESCRT-dependent protein sorting
Winter and Hauser, Trends in Plant Sc. 11

20. Ubiquitin

21. ESCRT-dependent vesicle formation
Figure 7.Immunogold detection of VSRs in wild-type and transgenic Arabidopsis plants with induced expression of ESCRT-III mutants. Six-day-old wild-type plants (A and B) and transgenic plants (C–J) germinated and cultured in the absence of inducers were transferred onto medium containing 10 μM β-estradiol for 24 h to induce the expression of VPS YFP (C and D), SNF7.1(L22W)-YFP (E and F), VPS YFP (G and H), or VPS YFP (I and J). Arabidopsis roots were subjected to high-pressure frozen/freeze substitution followed by ultrathin sectioning and immunogold labeling using VSR antibodies. Bar = 100 nm.
Cai et al., Plant Physiol. Vol.165 No.3c

22. FREE1 peripheral membrane-associated protein
binds to PI3P via the FYVE domain
Gao et al., Curr. Biol. 24

23. FYVE domain FYVE zinc finger domain
named after the Cys-rich proteins Fab1, YOTB, Vac1, EEA1
is composed of two small β-hairpins (zinc knuckles)
followed by a α-helix
binds PI3P
FYVE finger binds to two Zn-ions

24. FREE1 function transposon insertion in A.thaliana
homozygous free1 mutants are not viable
expression of GFP-tagged FREE1 rescued the seedling lethality
Gao et al., Curr. Biol. 24

25. FREE1 function
Gao et al., Curr. Biol. 24