1. Effects of salt on growth of P. fluorescens Alan Bohn and Liesel Hannes November 30, 2011 MB495 - Brown

2. How do salts affect growth of P. fluorescens? Common Anions and Cations Culture Density Lag phase Biphasic growth

3.  Culture density independent of salt choice at low concentrations NaCl

4.  Magnesium has a negative effect as a cation  Inhibits Maximum culture density and casues biphasic growth

5.  Biphasic Growth not a artifact of overnight culture

6.  Magnesium has a negative effect as a cation  Inhibits maximum culture density and causes biphasic growth

7.  Chloride ion in NaCl does not have a negative effect

8.  Bromide ion has a severe negative effect

9. Summary of Conclusions  Culture density independent of salt choice at low concentrations  Magnesium cation salts have a negative effect on culture density  Bromide anion has a negative effect on culture density

10. Future Directions Investigate the biphasic growth observed in MgSO 4 and MgCl 2 salts Calculate ideal concentrations of the different salts Test additional salts Salt requirements under anaerobic conditions

11. Questions?