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CHMI 2227 - E.R. Gauthier, Ph.D. 1 CHMI 2227E Biochemistry I Nucleic acids: - replication
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CHMI 2227 - E.R. Gauthier, Ph.D.2 DNA replication Nucleus DNA
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CHMI 2227 - E.R. Gauthier, Ph.D.3 DNA replication 5’AGCTAGCTGATATCGCGATCG3’ 3’TGCATCGACTATAGCGCTAGC5’ 5’AGCTAGCTGATATCGCGATCG3’ 3’TGCATCGACTATAGCGCTAGC5’ 5’AGCTAGCTGATATCGCGATCG3’ 3’TGCATCGACTATAGCGCTAGC5’
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CHMI 2227 - E.R. Gauthier, Ph.D.4 DNA replication
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CHMI 2227 - E.R. Gauthier, Ph.D.5 Semi-conservative Conservative Dispersive Non-conservative DNA replication 1) Conservation of the parental strands
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CHMI 2227 - E.R. Gauthier, Ph.D.6 DNA replication 1) The Meselson and Stahl experiment PNAS 1958;44;671-682
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CHMI 2227 - E.R. Gauthier, Ph.D.7 DNA replication 2) Directionality of replication Replication forks
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CHMI 2227 - E.R. Gauthier, Ph.D.8 DNA replication The replication fork 3’ Replication fork 5’ 3’ 5’ 3’ 5’ Direction of the replication fork
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CHMI 2227 - E.R. Gauthier, Ph.D.9 DNA replication Large DNA molecules can have multiple origins of replication
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CHMI 2227 - E.R. Gauthier, Ph.D.10 DNA replication 2) Directionality of replication 5’ 3’ 5’ 3’ 5’
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CHMI 2227 - E.R. Gauthier, Ph.D.11 DNA replication 2) Directionality of replication
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CHMI 2227 - E.R. Gauthier, Ph.D.12 DNA replication 2) Directionality: Okazaki fragments
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CHMI 2227 - E.R. Gauthier, Ph.D.13 DNA replication The replication fork
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CHMI 2227 - E.R. Gauthier, Ph.D.14 DNA replication 3) Nature of the replication machinery
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CHMI 2227 - E.R. Gauthier, Ph.D.15 DNA replication 3) DNA polymerase
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CHMI 2227 - E.R. Gauthier, Ph.D.16 DNA replication 3) DNA polymerase
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CHMI 2227 - E.R. Gauthier, Ph.D.17 DNA replication 3) DNA polymerase
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CHMI 2227 - E.R. Gauthier, Ph.D.18 DNA replication 3) DNA polymerase III of E. coli
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CHMI 2227 - E.R. Gauthier, Ph.D.19 DNA replication 3) DNA polymerase III of E. coli (Clamp loader) (DNA polymerase)
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CHMI 2227 - E.R. Gauthier, Ph.D.20 DNA replication 3) DNA polymerase III of E. coli http://oregonstate.edu/instruction/bb492/figletters/FigG1.html
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CHMI 2227 - E.R. Gauthier, Ph.D.21 DNA replication 3) DNA polymerase III of E. coli Sliding clamp Put DNA Here! Direction of replication
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CHMI 2227 - E.R. Gauthier, Ph.D.22 DNA replication 3) Primase
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CHMI 2227 - E.R. Gauthier, Ph.D.23 DNA replication Initiation at the origin of replication
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CHMI 2227 - E.R. Gauthier, Ph.D.24 DNA replication Leading strand synthesis http://oregonstate.edu/instruction/bb492/figletters/FigH4.html Unwinds DNA
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CHMI 2227 - E.R. Gauthier, Ph.D.25 DNA replication Lagging strand synthesis
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CHMI 2227 - E.R. Gauthier, Ph.D.26 DNA replication Lagging strand
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CHMI 2227 - E.R. Gauthier, Ph.D.27 DNA replication - DNA ligase Lacking Phosphodiester bond
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CHMI 2227 - E.R. Gauthier, Ph.D.28 DNA replication 4) Proofreading
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CHMI 2227 - E.R. Gauthier, Ph.D.29 DNA replication 4) Proofreading
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CHMI 2227 - E.R. Gauthier, Ph.D.30 DNA sequencing
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CHMI 2227 - E.R. Gauthier, Ph.D.31 PCR
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CHMI 2227 - E.R. Gauthier, Ph.D.32 PCR
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CHMI 2227 - E.R. Gauthier, Ph.D.33
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