1. Figure S1 Figure S1. Effect of SA on spore germination of M. oryae. The data presented were the means (± standard error) of spore germination from three independently biological experiments, which were examined by analysis of variance (ANOVA) to detect significant differences between means and those differing significantly (p<0.05) were compared using Duncan multiple range test (DMRT) at the same (5%) probability level using SPSS (Statistical Package for the Social Science) software package (Release v14.0; SPSS Company, USA). No significant difference (P <0.05) in spore germination was found. Supplementary materials for Li et al “Proteomic analysis of salicylic acid induced resistance to Magnaporthe oryzae in susceptible and resistant rice cultivars”

2. Figure S2. Effect of SA on mycelium growth of M. oryae. Fungal colonies were cultured on YS medium containing 0 (ck), 0.01, 0.1, 0.5, or 1 mM SA. Data bars were the means (± SE) of six replicates. Statistical analysis was carried out using the SPSS software. No significant difference (P <0.05) in colony diameters was found. Figure S2

3. Figure S3 A B

4. Figure S3. Quantitative analysis of the SA-modulated proteins in rice leaves at 12 h (A), 24 h (B) and 48 h (C). The protein intensity of the protein spots were calculated with PDQuest 8.0 software. Values are the means (±SE) of protein volumes on gels from three independent experiments. Statistical analysis was carried out using the SPSS software and the level of significance was calculated by Duncan’s multiple range test. Analysis of covariance (ANCOVA) was used to determine the significantly differentially expressed protein spots. The letters above the bars of the same protein spot indicate a statistically significant difference in protein expression at 0.01 level. “CK” indicates a control water treatment. “SA” indicates treatment with 0.1 mM SA. C

5. Figure S4. Identification of spot 17 by MALDI-TOF/TOF. The protein excised from CBB-staining gels was digested with trypsin, and the resulting peptides were analyzed using the 4700 Proteomic Analyzer. A, The MS spectra. The ion 1400.7 marked with an asterisk was analyzed by MS/MS. B, MS/MS spectra of ion 849.5. The protein was indentified as putative chaperonin 21 after database searching. Figure S4

6. Figure S5. Evolutionary relationships of endo-1,3-1,4-beta-glucanase (GenBank Q5UAW3), putative beta-1,3-glucanase (GenBank NP_001055377) and other 11 previously reported isozymes of rice glycosyl hydrolase Family 17. The evolutionary history was inferred using the Neighbor-Joining method (Saitou and Nei, 1987). The optimal tree with the sum of branch length = 10.67912644 is shown. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (1000 replicates) is shown next to the branches (Felsenstein, 1985). The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. The evolutionary distances were computed using the Poisson correction method (Zuckerkandl and Pauling (1965) and are in the units of the number of amino acid substitutions per site. All positions containing gaps and missing data were eliminated from the dataset (Complete deletion option). There were a total of 321 positions in the final dataset. Phylogenetic analyses were conducted in MEGA4 (Tamura et al., 2007). Figure S5