1. Waterborne Diseases Water Quality Coliform Counts Lactose + gas Extinction Dilution Most Probable Numbers – MPN EMB agar

2. Fecal Coliform Membrane Filter E. coli counts Enzyme substrate MUG IBGD 100 ml +/- Test

3. Wastewater 100-200 gal/day/person BOD Biochemical Oxygen Demand Water Quality Measure?

4. Sewage Treatment Primary Treatment Settling Tank Sludge Septic Tank

5. Secondary Treatments Aerobic Activated Sludge Trickling Filters

6. Anaerobic Treatment Sludge Digesters Methane Vented or Used?

7. Other Treatments Biodiscs Chlorine, Ozone, UV Tertiary treatment

8. Drinking Water Purification Potable Water Sedimentation Basin Alum Coagulation Sand Filter Chlorination

9. Cholera Vibrio cholerae 7 Pandemics 2 Biotypes El Tor & Classic O-1 Waterborne Diseases

10. Legionnaires Disease Philadelphia 1976 Legionella pneumophilia Erythromycin Complex Nutritional requirements Pontiac Fever

11. Protozoan Diseases Giardia lamblia Cysts 97% surface water Cryptosporidium parvum 1993 Milwaukee 403,000 cases

12. Other Waterborne Diseases Viruses Polio, Hepatitis Typhoid Fever Salmonella typhi Entamoeba histolytica Amoebic Dysentery Shigella dysenteriae