1. Supplemental figure 1 Adipocyte SVF E CD AB Supplemental figure 1: Impaired expression of Am2 and Ramps in obese mice (A-E) mRNA levels of Am2, Crlr, Ramp1, Ramp2 and Ramp3 in the adipocytes and stromal vascular fraction (SVF) of epididymal adipose tissue from db/db mice. n = 6. *P < 0.05 vs corresponding wild type (WT) mice. Am2 relative expression

2. Am2 Actb A AM2-TG WT Founder plasmid Southern Blot B AM2-TGWT AM2 β-actin C Supplemental figure 2 Supplemental figure 2: The adipose specific AM2 transgenic mice were established. (A) Southern blot identified adipocyte Am2 transgenic mice. (B) Reverse transcription polymerase chain reaction (RT-PCR) confirmed the overexpression of Am2 in adipose tissue. (C) Western blot analyzed increased expression of AM2 in adipose tissue. (D) Real time PCR analyses of Am2 expression in the white adipose tissue (WAT), macrophages (M ɸ ), liver, and skeletal muscle tissue (SKM). (E) Real time PCR analyses of Am2 expression in the adipocyte and stromal vascular fraction (SVF) of white adipose tissue. n=6, ***P<0.001 vs wild type (WT) mice. D E WTAM2-tg *** Am2 relative expression aAM2-tg

3. Supplemental figure 3 ChowHFDHFD+AM2 0 20 40 60 80 Serum triglycerides (mg/dl) ChowHFD 0 50 100 150 200 WTaAM2-tg Serum triglycerides (mg/dl) ChowHFD 0 50 100 150 WT aAM2-tg ** * Serum cholesterol (mg/dl) ChowHFDHFD+AM2 0 50 100 150 Serum cholesterol (mg/dl) ** C B A D Supplemental figure 3: The effects of AM2 on serum triglycerides and cholesterol levels. (A,B) The 10-week-old C57BL6 mice were fed with chow or HFD for 8 weeks, and subcutaneously infused with saline (vehicle) or AM2 (300 ngkg -1 h -1 ) by osmotic mini-pump for the latter 4 weeks. (A) serum total triglycerides and (B) total cholesterol levels were measured. (C,D) The 10-week-old aP2/AM2 transgenic mice and their WT littermates were fed with chow or high fat diet for 8 weeks. (C) serum total triglycerides and (D) total cholesterol levels were measured. n=4-10 per group, *P<0.05, **P<0.01.