1. Quantitative phase imaging, Photonics West, BiOS, San Francisco 2015 Věra Kollárová Quantitative phase imaging through scattering media Věra Kollárová, Jana Čolláková, Zbyněk Dostál, Tomáš Slabý, Pavel Veselý and Radim Chmelík Brno University of Technology, Czech Republic CEITEC – Central European Institute of Technology

2. Coherence Controlled Holographic Microscope (CCHM) - introduction Imaging through scattering layer - numerical simulation Imaging through scattering layer - experiment Outline Věra Kollárová

3. Coherence Controlled Holographic Microscope Věra Kollárová

4. Coherence Controlled Holographic Microscope Reference arm Object arm Source Specimen Věra Kollárová

5. CCHM and turbid medium Breast cancer cells in a phospholipid emulsion Věra Kollárová

6. Numerical simulation. Věra Kollárová Numerical simulation

7. Description of the CCHM imaging Věra Kollárová Numerical simulation

8. Image of the point in object arm (with diffuser). Mutual intensity (coherence properties) NA Imaging of a point object through a diffuser Image of the point shift s Věra Kollárová Numerical simulation

9. Diffuse layer Weak diffuser Strong diffuser Věra Kollárová Numerical simulation

10. Single point through a strong diffuser Shift Věra Kollárová Numerical simulation

11. 2D object through a diffuser - experiment Shift Věra Kollárová

12. Strong diffuser & effect of coherence Spatial coherence increase Věra Kollárová Numerical simulation

13. Experimental verification. Věra Kollárová

14. CCHM: phase object & weak diffuser. No diffuserWith diffuser 1 µm 1.5 µm 2 µm Věra Kollárová Experimental verification

15. . No diffuserWith diffuser CCHM: phase object & weak diffuser 1 µm 100 nm 50 nm Věra Kollárová Experimental verification

16. . CCHM: phase object & weak diffuser Věra Kollárová Experimental verification

17. . No shift CCHM: phase object & strong diffuser Věra Kollárová Experimental verification

18. . CCHM: phase object & strong diffuser Věra Kollárová Experimental verification

19. CCHM: living cells & scattering medium. Colorectal carcinoma cells under a 4 mm thick layer of 0.15% phospholipid emulsion. Start. After 3 hours. Věra Kollárová Experimental verification

20. Summary CCHM makes possible the quantitative phase imaging also through scattering media. The quality and details of the quantitative phase are dependent on the diffuser character and shift of images. The mutual shift of images can significantly improve quality the QPI. Věra Kollárová

21. Acknowledgement This work is supported by projects: EXCELLENT TEAMS (CZ 1.07/2.3.00/30.0005) from European Social Funds; GACR (15-14612S). Věra Kollárová

22. Thank you for your attention. Věra Kollárová