1. Author(s): Michael Hortsch, Ph.D., 2010 License: Unless otherwise noted, this material is made available under the terms of the Creative Commons Attribution – Non-Commercial 3.0 License: http://creativecommons.org/licenses/by-nc/3.0/ We have reviewed this material in accordance with U.S. Copyright Law and have tried to maximize your ability to use, share, and adapt it. The attribution key found at the end of the module provides information about how you may share and adapt this material. Copyright holders of content included in this material should contact open.michigan@umich.edu with any questions, corrections, or clarification regarding the use of content. For more information about how to cite these materials visit http://open.umich.edu/education/about/terms-of-use. Any medical information in this material is intended to inform and educate and is not a tool for self-diagnosis or a replacement for medical evaluation, advice, diagnosis or treatment by a healthcare professional. Please speak to your physician if you have questions about your medical condition. Viewer discretion is advised: Some medical content is graphic and may not be suitable for all viewers.

2. Histology of the Liver and Gallbladder - Second Look Michael Hortsch, Ph.D. Department of Cell and Developmental Biology University of Michigan © 2010 Michael Hortsch

3. The white lines indicate what kind of liver subdivision ? How is it defined ?

4. Starting at the bottom portal triad, indicate how blood is distributed in this liver lobule. Name the different vessels that participate in blood supply and drainage.

5. Identify the vessels that are marked by the colored arrows.

6. Name the vessels that are encircled by the different dashed lines.

7. How many portal triads do you see in this image ?

8. Name the structure indicated by the arrow.

9. How many central veins do you see in this image ?

10. Identify the opening that is encircled by the red dashed line. Identify the opening that is encircled by the green dashed line. What is the arrangement of liver cells called that is indicated by the blue dashed lines ?

11. Identify the cells that are marked by the different colored asterisks. * * * * * * *

12. Identify the cell outlined in this electron micrograph. List as many functions of this cell type as you can remember. Use this opportunity to review the appearance of cell organelles in high power electron micrographs (this micrograph is not the best for this purpose).

13. Name the area that is outlined by the red dashed lines. What cells, cellular structures and proteins do you expect to find in the space of Disse ? Name the cell that is outlined by the blue dashed lines. Image courtesy of Dorothy Sorenson, University of Michigan

14. Name the cell that is marked by the red asterisk. What general type of cell is this and what is its main function in the liver ? *

15. Find some Kupffer cells in the image on the left. Name some visible features that will help you to identify Kupffer cells.

16. Identify the cells that are indicated by the arrows.

17. This section of liver tissue was stained using the PAS method. The red stain in the hepatocyte cells is indicative of what substance ?

18. What is the area of liver tissue called that is indicated by the lines ? I II III Which area represents the location of hepatocytes that are most resistant to malnutrition ? How is it defined and why is it a useful concept ?

19. What is this pathological change called that is depicted in the image above ? Central vein

20. This is a special silver-stained section of a liver. Name the dark brown/black lines.

21. Identify the structure that is indicated by the red circle. Identify the two cells marked by the blue asterisks. * *

22. Name the cellular structure that is found in the liver tissue and depicted in this electron micrograph. What does the dark stain between the cells, which is indicated by the red arrows, represent ?

23. What is the main function of the gallbladder ? Identify the organ outlined by the green dashed line.

24. The gallbladder is lined by which type of epithelium ? Masson trichrome stained

25. Name a substance that controls the activity of the gallbladder muscularis externa. Identify the tissue type in the insert.

26. Slide 3:Michigan Histology 001_HISTO_40X.svs Slide 4:Michigan Histology 001_HISTO_40X.svs Slide 5:Michigan Histology 001_HISTO_40X.svs Slide 6:Michigan Histology 223 - Liver_001.svs portal triad 12% Slide 7:Michigan Histology 001_HISTO_40X.svs Slide 8:Michigan Histology 001_HISTO_40X.svs Slide 9:Michigan Histology 001_HISTO_40X.svs Slide 10:Michigan Histology 219 - Liver_001.svs central vein 12% Slide 11:Michigan Histology 220 - Liver_001.svs sinusoids 12% Slide 12:Michigan Histology 220 - Liver_001.svs Hepatocyte 55% Slide 13:Image courtesy of Dorothy Sorenson, University of Michigan Slide 14:Michigan Histology 220 - Liver_001.svs Kupffer cell 40% Slide 15:Michigan Histology 194_HISTO_40X.svs Slide 16:Michigan Histology 001_HISTO_40X.svs Slide 17:Image courtesy of Don McCallum Slide 18:Michigan Histology 001_HISTO_40X.svs Slide 19:Image courtesy of Don MacCallum (malaria infected hamster liver) Slide 20:Michigan Histology 198-1_HISTO_20X.svs Slide 21:Michigan Histology 223 - Liver_001.svs bile canaliculus 26% and 49% Slide 22:Michigan Histology 223 - Liver_001.svs bile duct 40% Slide 23:Michigan Histology 194_HISTO_40X.svs gall bladder appr 5% Slide 24:Michigan Histology Slide Collection Slide 195 trichrome 40x Slide 25:Michigan Histology 194_HISTO_40X.svs gallbladder wall 22% and 79% Attributions by Slide

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