1. P < 0.0005 n.s. WT 2d BKO 2d C WT: 13 images, KO: 14 images (1000x) 37.263.4

2. Based on TEM 2000x images (KO “D”, 10 images; HT “A”, 11 images) t-test: P < 0.003 10.717.1 Different animals from the same litter (2000x)

3. More from the same litter (1000x) (HT “A” & KO “E”) WT: ave of A & B (27 images) KO: ave of C & E (29 images) (B&C from the 1st slide)

4. E+ “I”E- “H” Ela-Cre 2d Toluidine Blue staining on 500nm sections At E18.5, ~70-80% of total pancreas is exocrine (--preliminary estimates on HE) At p1, ~50% of Perk gene is deleted in total pancreas of Ela-Cre If the exocrine part is 80%, deletion efficiency is actually 62.5% (~ close to the level at p11).

5. Semi-automatic estimation of ZG area, normalized to total exocrine area E(+)E(-) A 0.171 C 0.093 B 0.136 F 0.137 D 0.108 G 0.091 I 0.207 H 0.096 ave 0.16 ave 0.10

6. Global mixed background, 2d Total pancreas RNA --> real-time PCR (3 WT; 4 KO) On average, Pap: 2.4 fold increase in KO (but probably n.s.) Reg2: 7.9 fold increase in WT Bip: no change 2 KO had empty stomach One of them had the highest increase in both Pap and Reg2