1. AP Biology Lab #4: Plant Pigments and Photosynthesis
Carolina
Activity A: Leaf Pigment Chromatography

2. Purpose
To identify whether a green leaf is composed of one pigment or several. Also, to conduct a paper chromatography lab.

3. Materials Chromatography Jar (with solvent) Chromatography Paper
Spinach Leaf
Coin
Stapler
Ruler

4. Procedure Follow the lab instructions.
The solvent in the jar is very potent, keep capped and open and close quickly.
Roll the coin pressing firmly but not too hard.
The bands may be difficult to see.

5. Feedback Use Maple leaf (red and sugar) or Radicchio
Use several different species and compare
Look at Fall and Spring leaeves