1. 1 Growth and Cell Division Growth: –Not size, but number of cells Bacteria: –Binary fission Yeast: –Budding

2. 2 Cell Division

3. 3 Bacterial Growth Curve Lag phase Log phase Stationary phase Death phase

5. 5 Measuring Bacterial Concentration Serial dilution/ Standard plate counts CFU = Colony Forming Unit CFU/ml

8. 8 Measuring Bacterial Growth Direct microscopic counts

9. 9 Measuring Bacterial Growth Most probable number

12. 12 Measuring Bacterial Growth Filtration Turbidity (Spectrophotometry)

14. 14 Physical Factors Affecting Bacterial Growth pH Temperature

15. 15 Oxygen Moisture Physical Factors Affecting Bacterial Growth

16. 16 Osmotic pressure –Salt Radiation Physical Factors Affecting Bacterial Growth

17. 17 Nutritional Factors Affecting Bacterial Growth Carbon source Nitrogen source Sulfur/ phosphor Trace elements Vitamins Nutritional complexity Enzymes: –Extracellular –Periplasmic –Cytoplasmic

18. 18 Sporulation Endospores Other sporelike bacterial structures

19. 19 Culturing Bacteria — Streak Plates Streak plate method

20. 20 Culturing Bacteria — Pour Plates Pour plate method

21. 21 Culture Media Purposes Selective media Differential media Enrichment media

24. 24 Culture Conditions — O 2 Controlling oxygen content

25. 25 Culture conditions Maintaining cultures Preserved cultures Diagnostic tests Limitations