1. RT-PCR report: experiment test 1
Sample description:
Sample
isolation date
Lysate prep date
cell type
specimen age
passage
culture time
O2 (%)
equipment
Adeno
Flox
operator #
year
month
day 4
2014 1 19 29
chondrocyte
2 days
10 days 21
binder
LM/CM 5 6
RNA quantification after DNase treatment:
RNA integrity:
ARN sample #
[RNA] (ng/mL)
Vol. eq. 500ng RNA (mL)
Vol. eq mg RNA (mL)
Vol .eq mg RNA (mL)
sample 4
584.2
0.9
1.7
3.4
sample 5
543.3
1.8
3.7
sample 6
541.1
RT-PCR summary:
Summary of your RT-PCR experiment
RNA input (mg)
RT vol. (µl)
Dilution RT
Vol Dil RT (µl)
ng/well 1 20 50

2. RT-PCR report: experiment test 1
Plate map:

3. RT-PCR report: experiment test 1
Validation of the results: ACAN
-RT amplification curves:
+RT-RNA amplification curves:
No amplification: VALID
No amplification: VALID
+RT amplification curves:
+RT melting curves:
Amplification for all the samples: VALID
Single peak and homogenous Tm: VALID

4. RT-PCR report: experiment test 1
Validation of the results: COL2A1
-RT amplification curves:
+RT-RNA amplification curves:
Late amplification >>>+RT: VALID
No amplification: VALID
+RT amplification curves:
+RT melting curves:
Amplification for all the samples: VALID
Single peak and homogenous Tm: VALID

5. RT-PCR report: experiment test 1
Validation of the results: GAPDH
-RT amplification curves:
+RT-RNA amplification curves:
No amplification: VALID
No amplification: VALID
+RT amplification curves:
+RT melting curves:
Amplification for all the samples: VALID
Single peak and homogenous Tm: VALID

6. RT-PCR report: experiment test 1
Validation of the results: 18S
-RT amplification curves:
+RT-RNA amplification curves:
Late amplification >>> +RT!: ~VALID
Late amplification >>> +RT!: ~VALID
+RT amplification curves:
+RT melting curves:
Amplification for all the samples: VALID
Single peak and homogenous Tm: VALID

7. RT-PCR report: experiment test 1
Genes of reference distribution
GAPDH
18S Ct Ct
SEM =
SEM =
In these experimental conditions, GAPDH is more stable than 18S

8. RT-PCR report: experiment test 1
Analysis
ACAN
COL2A1
ACAN relative expression/GAPDH
COL2A1 relative expression/GAPDH
ACAN relative expression/18S
COL2A1 relative expression/18S