1. Microbiology Colony Morphology

2. Key Organisms for study  Gram negative organisms  PA - Pseudomonas aeruginosa  PV- Proteus vulgaris  EC- Escherichia coli  EA- Enterobacter aerogenes  Gram Positive Organisms  BS - Bacillus subtilis  SA - Staphylococcus aureus  SE - Staphylococcus epidermidis  SS- Streptococcus salivarius

3. Culture characteristics  Use supplement and handout to observe the growth of the four organisms in the slant, deep, broth, and on the plate.  Do the organisms look like one of the examples on your sheet?  Try to record their appearance on your templates

4. Colony morphology – Agar Plate

5. Margin of the colonies – The edge of colonies on the Agar Plate

6. Elevation( Observe from the side)

7. Colony morphology

8. Broth culture( refer to supplement)  Cloudy  Turbid( Flocculent)  Sediment formation ( may be due to tubes sitting in refrigerator)  Pellicle formation ( top)

9. Slants  Is there growth in the bottom( butt)?  Is there growth on the slant itself  What are the growth characteristics on the slant?  Filiform, echinulate, rhizoid.  Key words Aerobic Anaerobic Facultative

10. Part One – Completion of class 3 work  When you have finished observing all of your cultures  Place all tubes in rack in hazardous waste  Place all plates in cans in hazardous waste  Wipe down desk top