Presentation is loading. Please wait.

Presentation is loading. Please wait.

Synechococcus PCC7942 Prochlorococcus MED Prochlorococcus SS120 CC9311 CC9617 WH7803 WH7805 CC9318 CC9317 CC9305-3 PCC6307 E. lake isolate CC9616 CC9701.

Published byVirgil Bryant Modified over 9 years ago

Similar presentations

Presentation on theme: "Synechococcus PCC7942 Prochlorococcus MED Prochlorococcus SS120 CC9311 CC9617 WH7803 WH7805 CC9318 CC9317 CC9305-3 PCC6307 E. lake isolate CC9616 CC9701."— Presentation transcript:

1 Synechococcus PCC7942 Prochlorococcus MED Prochlorococcus SS120 CC9311 CC9617 WH7803 WH7805 CC9318 CC9317 CC9305-3 PCC6307 E. lake isolate CC9616 CC9701 SS9401 CC9605 CC9703 C129 WH8102 WH8103 WH8011 WH8112 CC9301 WH8113 CC9702 0.4 No PUB No PE 0.4 0.9 2.0 1.2 0.8 1.1 2.0 1.0 1.6 0.1 substitutions/site Motile isolates 100 92 78 74 95 94 Fig. 1 Neighbour-joining tree constructed using Jukes-Cantor corrected distances using a 612-bp fragment of the DNA- dependent RNA polymerase (rpoC1) gene sequence of different Synechococcus-like isolates. The values in boxes are PUB/PEB fluorescence ratios. (From Toledo et al., 1999).

2 Probe 645 HLI 3´-CTA GCC GAA CTC ATA CCA-5´ Target (E.coli 645-662) 5´-GAU CGG CUU GAG UAU GGU-3´ PCC9511/EQPAC1 (HLI).................. ENATL1 env.clone (HLI).................. ENATL4 env.clone (LL)... U.............. NATL1-MIT (LL)... U.............. TAK9803-2 (HLII)..A A.............. MIT9303/MIT9313 (LL)... U.........G.... SS120 (LL)..A G.............. Syn.WH7803/WH8103.U. G.........G.... Syn.WH8018.U. G....C....G.... Syn.WH7805.U. G...NC....G.... Probe 645 HLII 3´-CTT TCC GAA CTC ATA CCA-5´ Target (E.coli 645-662) 5´-GAA AGG CUU GAG UAU GGU-3´ TAK9803-2.................. PCC9511/EQPAC1..U C.............. ENATL1 env.clone..U C.............. ENATL4 env.clone..U U.............. NATL1-MIT..U U.............. MIT9303/MIT9313... U.........G.... SS120... G.............. Syn.WH7803/WH8103.UU G.........G.... Syn.WH8018.UU G....C....G.... Syn.WH7805.UU G...NC....G.... Probe 645 LL 3´-CTA ACC GAA CTC ATA CCA-5´ Target (E.coli 645-662) 5´-GAU UGG CUU GAG UAU GGU-3´ NATL1-MIT.................. ENATL4 env.clone.................. MIT9303/MIT9313.............G.... TAK9803-2..A A.............. PCC9511/EQPAC1... C.............. ENATL1 env.clone... C.............. SS120..A G.............. Syn.WH7803/WH8103.U. G.........G.... Syn.WH8018.U. G....C....G.... Syn.WH7805.U. G...NC....G.... Prochlorococcus-specific 16S rDNA oligonucleotides

3 181 HLI Prochlorococcus-specific probe Probe 181HLI 3´-TAC GGC TAT CCA CTT TAC-5’ Target(E.coli 181-198) 5´-AUG CCG ATA GGU GAA AUG-3´ PCC9511/EQPAC1.................. ENATL1 env.clone.................. ENATL4 env.clone.......G.......... NATL1-MIT/MIT9303/MIT9313.......G.......... SS120.......G.......... TAK9803-2.....U.CU......... Syn sp.WH7805/7803/8018.......G.......... Syn sp.WH8103...... CG....... … 181 LL Prochlorococcus-specific probe Probe 181LL 3´-TAC GGC TCT CCA CTT TAC-5´ Target(E.coli 181-198) 5´-AUG CCG AGA GGU GAA AUG-3´ NATL1-MIT.................. MIT9303/MIT9313.................. SS120.................. ENATL4 env.clone.................. Syn. sp.PCC6307.................. Syn.sp.WH7805/7803/8018.................. Syn.sp. Biwa-B0/-G0/-P0.................. Oscillatoria neglecta M-82.................. Syn.sp. WH8103...... C........... PCC9511/EQPAC1.......U.......... ENATL1 env.clone.......U.......... TAK9803-2.....U.CU...... … Prochlorococcus-specific 16S rDNA oligonucleotides

4 Prochlorococcus MIT9303/MIT9313/MIT9211 LL specific probe Probe 148LLMIT 3´-CTA TTG CCA ACC TTT GCC-5’ Target(E.coli 148-166) 5´-GAU AAC GGU UGG AAA CGG-3´ MIT9211/MIT9303/MIT9313.................. PCC9511/EQPAC1.................A MIT9107/MIT9302/MIT9312.................A SS120.................A TAK9803-2.................A Syn sp.WH8103.................A Syn sp.WH7805........C......... ‘GENERAL’ Prochlorococcus probe Probe 405PRO 3´-ACT CCC TGC TTC CGG AGA-5´ Target(E.coli 405-422) 5´-UGA GGG ACG AAG GCC UCU-3´ PCC9511/EQPAC1.................. NATL1-MIT.................. TAK9803-2.................. SS120.................. ENATL1 env.clone.................. ENATL4 env.clone.................. MIT9303/MIT9313.......U.......... Syn.sp. WH7803/7805/8103.......U..........

5 Phylogenetic relationships among marine Prochlorococcus & Synechococcus strains

6 Phylogenetic relationships among marine Synechococcus strains

7 Phylogenetic relationships among marine Synechococcus strains

8 Comparison of gene clusters coding for phycobiliproteins Prochlorococcus marinus SS120 mpeB A CU cpeZYorf140 cpeAB mpeV orfs 200,236,257 rpcB A orf 196 rpcE F Synechococcus WH 8020 14891 bp 10153 bp uvrD cpeB A Z mpeX Y Orf195,181 ppeC orf431 metK Prochlorococcus marinus MED4 uvrD cpeB Orf181 metK 2850 bp

9 Phylogenetic relationships amongst Prochlorococcus strains 0.1 Prochlorococcus sp. HL1SB3 (RCC283) Prochlorococcus sp. MED4 (RCC153) Prochlorococcus sp. EQPAC1 (RCC278) Prochlorococcus sp. HL1GP2 (RCC281) Prochlorococcus sp. TATL4 Prochlorococcus sp. PCC9511 Prochlorococcus sp. EQPAC2 (RCC279) Prochlorococcus sp. TATL1A Prochlorococcus sp. NATL2 SAR6 Prochlorococcus sp. SB Prochlorococcus sp. TATL2 Prochlorococcus sp. MIT9302 Prochlorococcus sp. MIT9312 Prochlorococcus sp. GP2 Prochlorococcus sp. TATL1B Prochlorococcus sp. MIT9107 Prochlorococcus sp. TAK9803 (RCC264) Prochlorococcus sp. NATL2A Prochlorococcus sp. MITNATL2 (RCC280) Prochlorococcus sp. PAC1 Prochlorococcus sp. MITNATL1 (RCC277) Prochlorococcus sp. SS120 (RCC156) SAR7 Prochlorococcus sp. MIT9303 Prochlorococcus sp. MIT9313 SAR139 SAR100 Synechococcus WH8103 Synechococcus WH7805 Synechococcus WH8101 Synechococcus PCC6307 Microcystis elabens Synechococcus PCC 6301 Oscillatoria corallinae HLI HLII LL

10 Oligonucleotide HRP-labeled C6 amino-link Samples on filters or on glass slides Observe under fluorescence microscope Hybridisation Washing Equilibriation in buffer Incubation with fluorochrome-tyramide 35 °C46 °C 48 °C35 °C Fluorescent in situ hybridisation Monolabeled-fluorochrome Fluorescein, Rhodamine, CY3, BODIPY PROBE LABELING HYBRIDISATION

Download ppt "Synechococcus PCC7942 Prochlorococcus MED Prochlorococcus SS120 CC9311 CC9617 WH7803 WH7805 CC9318 CC9317 CC9305-3 PCC6307 E. lake isolate CC9616 CC9701."

Similar presentations

Translation By Josh Morris.

Translation By Josh Morris.
 -GLOBIN MUTATIONS AND SICKLE CELL DISORDER (SCD) - RESTRICTION FRAGMENT LENGTH POLYMORPHISMS (RFLP)

 -GLOBIN MUTATIONS AND SICKLE CELL DISORDER (SCD) - RESTRICTION FRAGMENT LENGTH POLYMORPHISMS (RFLP)
ATG GAG GAA GAA GAT GAA GAG ATC TTA TCG TCT TCC GAT TGC GAC GAT TCC AGC GAT AGT TAC AAG GAT GAT TCT CAA GAT TCT GAA GGA GAA AAC GAT AAC CCT GAG TGC GAA.

ATG GAG GAA GAA GAT GAA GAG ATC TTA TCG TCT TCC GAT TGC GAC GAT TCC AGC GAT AGT TAC AAG GAT GAT TCT CAA GAT TCT GAA GGA GAA AAC GAT AAC CCT GAG TGC GAA.
Supplementary Fig.1: oligonucleotide primer sequences.

Supplementary Fig.1: oligonucleotide primer sequences.
Gene Mutations Worksheet

Gene Mutations Worksheet
Transcription & Translation Worksheet

Transcription & Translation Worksheet
Transcription and Translation

Transcription and Translation
IGEM 8.3.10 - 8.6.10. Arsenic Bioremediation Possibly finished biobrick for ArsR by adding a RBS and terminator. Will send for sequencing today or Monday.

IGEM Arsenic Bioremediation Possibly finished biobrick for ArsR by adding a RBS and terminator. Will send for sequencing today or Monday.
Proteins are made by decoding the Information in DNA Proteins are not built directly from DNA.

Proteins are made by decoding the Information in DNA Proteins are not built directly from DNA.
Nature and Action of the Gene

Nature and Action of the Gene
FEATURES OF GENETIC CODE AND NON SENSE CODONS

FEATURES OF GENETIC CODE AND NON SENSE CODONS
Undifferentiated Differentiated (4 d) Supplemental Figure S1.

Undifferentiated Differentiated (4 d) Supplemental Figure S1.
Supplemental Table S1 For Site Directed Mutagenesis and cloning of constructs P9GF:5’ GAC GCT ACT TCA CTA TAG ATA GGA AGT TCA TTT C 3’ P9GR:5’ GAA ATG.

Supplemental Table S1 For Site Directed Mutagenesis and cloning of constructs P9GF:5’ GAC GCT ACT TCA CTA TAG ATA GGA AGT TCA TTT C 3’ P9GR:5’ GAA ATG.
Lecture 10, CS5671 Neural Network Applications Problems Input transformation Network Architectures Assessing Performance.

Lecture 10, CS5671 Neural Network Applications Problems Input transformation Network Architectures Assessing Performance.
GeneGenbank NumberSequences GFPU55762 Sense: 5’-GUU CAG CGU GUC CGG CGA GTT Antisense: 5’-CUC GCC GGA CAC GCU GAA CTT Rat TLR2NM_198769 Sense: 5’-GGA UCU.

GeneGenbank NumberSequences GFPU55762 Sense: 5’-GUU CAG CGU GUC CGG CGA GTT Antisense: 5’-CUC GCC GGA CAC GCU GAA CTT Rat TLR2NM_ Sense: 5’-GGA UCU.
Fig. S1 siControl E2 G1: 45.7% S: 26.9% G2-M: 27.4% siER  E2 G1: 70.9% S: 9.9% G2-M: 19.2% G1: 57.1% S: 12.0% G2-M: 30.9% siRNF31 E2 A B siRNF31 siControl.

Fig. S1 siControl E2 G1: 45.7% S: 26.9% G2-M: 27.4% siER  E2 G1: 70.9% S: 9.9% G2-M: 19.2% G1: 57.1% S: 12.0% G2-M: 30.9% siRNF31 E2 A B siRNF31 siControl.
GENE EXPRESSION. Gene Expression Our phenotype is the result of the expression of proteins Different alleles encode for slightly different proteins Protein.

GENE EXPRESSION. Gene Expression Our phenotype is the result of the expression of proteins Different alleles encode for slightly different proteins Protein.
PART 1 - DNA REPLICATION PART 2 - TRANSCRIPTION AND TRANSLATION.

PART 1 - DNA REPLICATION PART 2 - TRANSCRIPTION AND TRANSLATION.
RNA Structure Like DNA, RNA is a nucleic acid. RNA is a nucleic acid made up of repeating nucleotides.

RNA Structure Like DNA, RNA is a nucleic acid. RNA is a nucleic acid made up of repeating nucleotides.
TABLE 2 Oligonucleotide primers and probes used in FISH* S1PRO634RHLIProchlorococcus GCC GAT CAG TTT CCA CTG S2PRO634RHLIIProchlorococcus GCC TTT CAG.

TABLE 2 Oligonucleotide primers and probes used in FISH* S1PRO634RHLIProchlorococcus GCC GAT CAG TTT CCA CTG S2PRO634RHLIIProchlorococcus GCC TTT CAG.

Similar presentations

Ads by Google