1. Seminar assignment Handed out at course start Group work/individual assessment Research based aspects, requires independent work Obtain appropriate information - lectures, discussions, web, articles..… Time scheduled to prepare answer Presentation of assignment in seminar format: Groups 3, 4, 5 + 6Friday 19th November Groups 1 + 2Thursday 25th November Grading = pass / fail

2. Seminar assignment Why? Training for the exam Attain “core” knowledge Apply knowledge from previous courses Sort out misunderstandings

3. 1. How could lipid accumulation in the liver be due to defects in adipose tissue? Seminar assignment Cusi K. Curr Diab Rep (2010) 10: 306-315

4. Seminar assignment Association between liver fat accumulation and a single nucleotide polymorphism (SNP) located in the promoter of gene X in a putative binding site for peroxisome proliferator activated receptors (PPARs). 2. Describe the molecular mechanism of action of PPARs

5. Seminar assignment 3. Calculate the frequency of each of the alleles

6. Seminar assignment Your task is to design three complementary research studies in order to explore the medical importance of the SNP Study 1. A confirmative human study of your preliminary findings that is population based (~5000 individuals). Study 2. An individually based human study consisting of ~50 individuals that could test whether drugs could influence the degree of hepatosteatosis depending on the SNP in gene X Study 3. Design and describe an in vitro study that will investigate the putative underlying molecular mechanisms using commercially available cell lines

7. Seminar assignment Handed out at course start Group work/individual assessment Research based aspects, requires independent work Obtain appropriate information - lectures, discussions, web, articles..… Time scheduled to prepare answer Presentation of assignment in seminar format: Groups 3, 4, 5 + 6Friday 19th November Groups 1 + 2Thursday 25th November Grading = pass / fail

8. Time in schedule for Groups 3 + 4 to work with seminar assignment

9. Group 3: 9.00-10.30 Addison Group 4: 10.30-12.00 Addison

10. Time in schedule for Groups 5 + 6 to work with seminar assignment

11. Group 5: 9.00-10.30 Einthoven Group 6: 10.30-12.00 Einthoven

12. Time in schedule for Groups 1 + 2 to work with seminar assignment

13. Group 1: 10.00-11.30 Addison Group 2: 10.00-11.30 Einthoven

14. Seminar assignment On demand – support session Wednesday Nov 10, 3 pm Register before Tuesday Nov 9 to: sofia.asp@ki.se and ewa.ehrenborg@ki.se Hand in specific questions What it is that needs to be clarified