1. Extracellular Molding of Invasive and Non-invasive Cancer Cells Elizabeth Smith Mentor: Brandon Lutz Advisor: Dr. Michael Cho Program Director: Dr. Andreas Linninger RET, University of Illinois-Chicago NSF CBET EEC-0743068 June 30, 2011

2. Background 1) Cells have different morphologies NCM460 Breast CancersColon Cancers Invasive Non-Invasive SW620

3. Background 2) Cells respond to extracellular topography OsteocytehMSCAdipocyte Substrate rigidity differencesSubstrate topography differences

4. Goals Current Provide a microscale mold of differing cell morphology to alter the morphology of the seeded cells Future Determine if the change in morphology leads to change in cell behavior

5. Noninvasive Cancer Cell Invasive Cancer Cell Glass Substrate PDMS Substrate Current Goal Provide a microscale mold of differing cell morphology to alter the morphology of the seeded cells Future Determine if the change in morphology leads to change in cell behavior

6. Experimental Outline

7. Expected Outcomes Invasive CellsNon-Invasive Cells Grown on non-invasive molds become Grown on invasive molds become

8. Applications Micro-scale texturing of… Implant technology Breast reconstruction, bone & tooth, etc. On-site cancer suppression Stent technology Invasive cell “catcher”

9. References Information Curtis, A., & Wilkinson, C. (January 01, 1997). Topographical control of cells. Biomaterials, 18, 24, 1573. Engler, A. J., Sen, S., Sweeney, H. L., & Discher, D. E. (January 01, 2006). Matrix Elasticity Directs Stem Cell Lineage Specification. Cell, 126, 4, 677. Lim, J. Y., & Donahue, H. J. (January 01, 2007). Cell Sensing and Response to Micro- and Nanostructured Surfaces Produced by Chemical and Topographic Patterning. Tissue Engineering, 13, 8, 1879-1891. Images http://www.cardiolabel.com/stent%202.jpg http://media.wiley.com/wires/WNAN/WNAN74/nfig007.jpg http://www.genengnews.com/media/images/GENHighlight/Aug2_2010_FromPR_ HumanMesenchymalStemCellGrowingOnMicroposts_NewInsightsonHowTheyBe comeTissues_crop1351984273.jpg http://www.genengnews.com/media/images/GENHighlight/Aug2_2010_FromPR_ HumanMesenchymalStemCellGrowingOnMicroposts_NewInsightsonHowTheyBe comeTissues_crop1351984273.jpg http://www.riverinahipandknee.com.au/Research.aspx

10. Thank You