1. SG KB 2009 NIGMS Workshop: Enabling Technologies for Structural Biology Section on Structural Analysis Helen M. Berman March 4, 2009 How to use the PSI Structural Genomics Knowledgebase for Research Part I: Introduction

2. Knowledgebase The PSI Structural Genomics Knowledgebase will turn the products of the PSI effort into major advances in knowledge that can be used to understand living systems and human disease.

3. Knowledgebase Goals It will provide a “marketplace of ideas” that  connects protein sequence information to 3D structures and homology models  enhances functional annotations  provides access to new experimental protocols and materials It will enable advancements in structural genomics  by communicating and providing visibility and accessibility of information and technology advances of the PSI  through presentation and discussion of the most provocative challenges with the general community  by fostering community collaborations

4.  To capture, make accessible, and highlight elements of the high- throughput pipelines for use by various scientific communities  To leverage such information through the generation of molecular models and functional annotation Scope Genomic Based Target Selection Data Collection Structure Determination Isolation, Expression, Purification, Crystallization PDB Deposition & Release Models Annotations Publications Metrics Technology Experimental Tracking Target Selection Materials

5. Knowledgebase Users  Biologists  Biochemists  Functional Genomicists  Pharmacologists  Bioinformatics  Chemists  Clinical Researchers and Physicians  Teachers and Students

6. Navigating the PSI SGKB Homepage  Database searchable by sequence, text, and PDB ID and delivers aggregate reports, inventories  Links to PSI projects, external resources, and publications  Link to central Community- Nominated Targets Proposal system  SG Gateway with Nature delivers research findings, technologies, news and events related to the PSI and structural genomics  Publicizes recently solved PSI structures or new editorial content

7. Tour of the PSI SGKB In today’s tutorial, we will demonstrate:  How to navigate the site and its interactive features  How to search the PSI SGKB for information  How to find walkthroughs and exercises about this site

8. Acknowledgements KB GroupPSI Resources Wendy TaoAndrei Kouranov (Exp. Data Tracking) Raship ShahTorsten Schwede (Models) James ChunPaul Adams (Technology) Margaret Gabanyi Josh La Baer (Materials) John WestbrookWladek Minor (Publications) Access Information http://kb.psi-structuralgenomics.org Nature Matthew Day Boyana Konforti SG KB PSI SGKB Steering Committee Eaton E. Lattman, Chair