1. CASIROZ – participant 8 assistant contractor to participant 7 Michael Tausz, Dieter Grill Maria Müller Karin Herbinger, Klaus Remele Institut für Pflanzenphysiologie, Universität Graz, Schubertstraße 51, A-8010 Graz, Austria

2. Planned investigations Biochemical defense –Ascorbate system –Glutathione system –Tocopherol –Chloroplast pigments Chromosomal damages –Aberration rates

3. Asc DHAsc GSH GSSG NADPH DHAsc Asc Cell wall Plasmalemma O3O3 ROS Symplast Apoplast Chloroplast NADP GSH GSSG NADPHAsc NADP DHAsc Secondary products Secondary products Photosynthetic apparatus ROS

5. Aberration types –sticky chromosomesanaphase bridges

6. Further aberrations –vagrant chromosomes in –metaphaseanaphase

7. Planned experiments Biochemical defense –Ascorbate system –Glutathione system –Tocopherol –Chloroplast pigments Chromosomal damages –Aberration rates

8. Experiments Container exposed young trees Branch cuvette exposures Mature trees

9. Container exposed young trees Ascorbate; glutathione; tocopherols; pigments in leaves of young (3-yrs-old) trees (exposed at 2 canopy levels). Ascorbate; glutathione; tocopherols in roots of young (3-yrs-old) trees (exposed at 2 canopy levels). Depends on available material - possible only once per season! Chromosomal aberration analysis in roots of young (3-yrs-old) trees (exposed at 2 canopy levels).

10. Branch cuvette exposures Ascorbate; glutathione; tocopherols; pigments in leave samples included in branch cuvette fumigation systems (interface to partner 7).

11. Mature trees Tocopherols and chloroplast pigments in leaves of adult trees (interface to partner 4 who contributes glutathione and ascorbate). Sampling schedule partner 4. Ascorbate; glutathione; tocopherols in roots of adult trees. Sampling schedule depending on available root material - possibly only once a season. Chromosomal aberration analysis in roots of adult trees.