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Published byNelson Ferguson Modified over 9 years ago
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DNA library Genomic libraryenomic library cDNA library Genomic Library: DNA fragments representing the entire genome of an organism. cDNA Library :Complementary DNA molecules synthesized from mRNA molecules in a cell. All mRNA present in cell Collection of cloned DNA fragments
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Isolation of RNA Isolated Cytoplasmic RNA consists of ribosomal RNAs (rRNAs)ribosomal RNAs transfer RNAs (tRNAs).transfer RNAs mRNAs with 3′ poly(A) tails
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3′ poly(A) tails of mRNA hybridize to oligo-dT covalently coupled to the column matrix
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Preparation of cDNA from mRNA
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EcoRI methylase, a modification enzyme that catalyzes addition of a methyl group to two adenines in the EcoRI recognition sequence. The methylated restriction site is not cleaved by EcoRI, assuring that a cell making this restriction enzyme does not destroy its own DNA.enzyme
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