1. 1 SURVEY OF BIOCHEMISTRY Protein-NA Interactions DNA Replication, Damage, and Repair

2. 2 What to Know for Exam 1 Structures: –NA, AA, Classifications –Base pairs, numbering system –Polypeptide backbone –Dihedral angles Characterization –Beer’s Law –Protein Purification and SDS-PAGE –pI Processes: General Concepts

3. 3 Study Tips Read text Review Lecture Notes Practice problems Read text again

4. 4 PRS Which of the following reagents would be suitable for reducing a disufide bond? 1.Methionine 2.2-Mercaptoethanol 3.Sodium Dodecyl Sulfate 4.Cysteine

5. 5 PRS Question The dihedral angle psi is defined by which of the following atoms? 1.N-C alpha -C carbonyl -N 2.C alpha -C carbonyl -N-C alpha 3.C carbonyl -N-C alpha -C carbonyl 4.N-C alpha -C carbonyl =O

6. 6 PRS Question How many H-bond acceptors in adenine participate in Watson- Crick H-bonds with thymine? 1.None 2.One 3.Two 4.Three

7. 7 PRS Question How many H-bond acceptors in adenine participate in Watson- Crick H-bonds with thymine? 1.None 2.One 3.Two 4.Three

8. 8 Protein-DNA Interactions Restriction Enzymes Repressor Proteins Transcription Factors –Zinc Fingers –Leu Zippers Eukaryotic Chromosomes Specific recognition Nonspecific recognition

9. 9 Restriction Enzymes H bonds stabilize complex DNA binds in cleft formed by 2 subunits EcoRI TCGCGAATTCGCG

10. 10 Helix-Turn-Helix Motif

11. 11 Zinc Fingers C2H2C4C6C2H2C4C6 http://www.web-books.com/MoBio/Free/Ch4F2.htm

12. 12 Regulatory Protein in Yeast

13. 13 Leu Zippers

14. 14 Leu Zippers

15. 15 DNA Replication

16. 16 DNA Replication

17. 17 DNA Replication is semidiscontinuous

18. 18 DNA Damage

19. 19 DNA Repair

20. 20 DNA Repair