1. + Introduction to the Eukaryotic Cell Exercise 9

2. + Announcements Students have time to observe Week 8 plates and finish their LNAs. Post Lab 9/Pre Lab 10 are due by the time your lab meets next. LNA Eukaryotic Cell is due next week in lab.

3. + Goals To learn the basic structures associated with eukaryotic cells. To identify the differences between animal and plant cells. To understand the basis of cell culture, and the changes imposed on cells under culture conditions. To understand the techniques of fluorescence and electron microscopy and cell culture.

4. + Animal Cell

5. + Plant Cell

6. + The Exercise Part I: Plant cells: Onion epidermis Basic cell structure of a plant lacking chloroplasts Iodine: observe nucleolus Janus Green: observe mitochondria

7. + Part II: Animal cells: Human squamous epithelium Observe differences from the plant cells Do the mitochondria vary in size from those found in plant cells? The Exercise

8. + Part III: Autofluorescence View Tradescantia under the fluorescent microscope Chlorophyll pigments autofluoresce The Exercise

9. + Part IV: Anacharis Leaf Cells View a living plant cell with chloroplasts The Exercise

10. + Part V: Electron Micrographs and Cellular Infrastructure View EM transparencies Identify organelles You may want to take notes for future identification (i.e. Exam 2 and Final) The Exercise

11. + Be Able to Identify: Nucleus Nuclear envelope Nuclear pore Nucleolus Rough Endoplasmic Reticulum Ribosome Mitochondria

12. + Be Able to Identify: Golgi complex section viewtop view

13. + Golgi top view

14. + Golgi section view

15. + Be Able to Identify: Mitochondrion

16. + Be Able to Identify: Nucleus Vacuole Chloroplast

17. + Be Able to Identify: Thylakoid

18. + Be Able to Identify: Plasma Membrane

19. + Summary of Exercise 9 Part I: Onion epidermis slide x 3: water / Iodine / Janus Green size, nucleus, vacuole, cell wall/membrane, chloroplast, mitochondria (?) Part II: Cheek cells slide x 2: water / Janus Green size, nucleus, cell membrane, mitochondria (?) Part III: Tradescantia TA Autofluorescence Part IV: Anacharis Cytoplasmic streaming