2. Cellular Respiration Part 2 Producing ATP by Oxidative Phosphorylation Energy from Macromolecules

3. Releasing Energy From Glucose Glucose (6C) 2 X Pyruvate (3C) 2 X Acetyl-CoA (2C) Cytoplasm Mitochondrion Citric Acid Cycle ATP e - Carriers Electron Transport Chain ½ O 2 H2OH2OH2OH2OATP 2 H + 4 CO 2 2 CO 2 2 CO 2 ATP Glycolysis Energy Released e-e-e-e- e-e-e-e- e-e-e-e- O 2 present

4. Locations of Cellular Respiration Components Matrix: Citric Acid Cycle and Pyruvate Oxidation Mitochondrion A Crista Inner Membrane: Has ATP Synthase Electron Transport Chain Intermembrane Compartment H + accumulates a b Outer Membrane

5. Sequence of Electron Carriers The poison cyanide prevents transfer of electrons to oxygen NADH donates electrons to Complex I ( NADH Dehydrogenase) FADH 2 donates electrons to Complex II (Succinate Dehydrogenase) Cytochromes are electron carriers with a heme prosthetic group

6. Formation of H + Gradient The poison arsenic prevents the buildup of the H + gradient Protons are pumped from the matrix into the intermembrane space Flow of protons through ATP synthase powers ATP production

7. ATP Synthase H + ions cause the rotor of ATP synthase to spin Sites in the catalytic knob are activated to catalyze ATP production The inner mitochondrial membrane is impermeable to H +, which can only pass through the ATP synthase The internal rod also spins as a result of rotor movement

8. Oxidative Phosphorylation Production of ATP as a result of electron transfer through carriers in the Electron Transport ChainProduction of ATP as a result of electron transfer through carriers in the Electron Transport Chain –Electrons pass through a set of membrane-associated carriers by a series of redox reactions –Energy from electron transport powers the active transport of H + to the intermembrane compartment of the mitochondrion, building a concentration gradient –Chemiosmosis: Diffusion of hydrogen ions (H + ) through the differentially permeable inner mitochondrial membrane, resulting in ATP production H + can only cross the membrane into the mitochondrial matrix through the pores of an ATP-synthesizing enzymeH + can only cross the membrane into the mitochondrial matrix through the pores of an ATP-synthesizing enzyme Movement of H + through the enzyme provides energy for ATP synthesisMovement of H + through the enzyme provides energy for ATP synthesis

9. Applying Your Knowledge A.Where is ATP Synthase located? B.Where do H + ions build up as a result of active transport? C.Where are the carriers of the electron transport chain located? 1. Inner Mitochondrial Membrane 2. Mitochondrial Matrix 3. Outer Mitochondrial Membrane 4. Intermembrane space

10. Fermentation Glucose (6C) 2 X Pyruvate (3C) 2 X Acetyl-CoA (2C) Cytoplasm Mitochondrion Citric Acid Cycle ATP e - Carriers 4 CO 2 2 CO 2 2 CO 2 ATP Glycolysis oxidized e - Carriers Fermentation 2 X Lactate (3C) (in muscle) O 2 present when O 2 becomes available X O 2 absent

11. Alcoholic and Lactic Acid Fermentation Muscle cells Microorganisms Yeasts Some Plants Alcoholic Fermentation Lactic Acid Fermentation

12. Applying Your Knowledge A.Muscle cells produce lactate B.Muscle cells produce ATP C.Yeast cells produce ethanol 1. In the Presence of Oxygen 2. In the Absence of Oxygen 3. Either in the presence or absence of Oxygen

13. Energy From Macromolecules Glucose (6C) 2 X Pyruvate (3C) 2 X Acetyl-CoA (2C) Citric Acid Cycle Glycolysis Monosaccharides Polysaccharides Disaccharides Gluconeogenesis

14. Energy From Macromolecules Glucose (6C) 2 X Pyruvate (3C) 2 X Acetyl-CoA (2C) Triglycerides Fatty Acids multiples of 2C Glycerol (~5%) Citric Acid Cycle Glucose (6C) 2 X Pyruvate (3C) GlycolysisGluconeogenesisDAP

15. Energy From Macromolecules 2 X Pyruvate (3C) 2 X Acetyl-CoA (2C) Proteins Other amino Acids 3C-amino acids Citric Acid Cycle Glucose (6C) GlycolysisGluconeogenesis

16. Applying Your Knowledge A.Muscle cells use fatty acids for energy B.Muscle cells use glucose or glycogen for energy 1. In the Presence of Oxygen 2. In the Absence of Oxygen 3. Either in the presence or absence of Oxygen

17. Applying Your Knowledge A.Brain cells use glucose for energy B.Brain cells use proteins for energy 1. In the Presence of Carbohydrates 2. In the Absence of Carbohydrates 3. Either in the presence or absence of Carbohydrates

18. Anabolic Interconversions Glucose (6C) 2 X Pyruvate (3C) 2 X Acetyl-CoA (2C) Citric Acid Cycle GlycolysisPolysaccharidesGluconeogenesis Fatty Acids Glycerol Amino Acids Triglycerides Proteins

19. Regulation of Glycolysis Phosphofructokinase isPhosphofructokinase is –allosterically inhibited by ATP –allosterically activated by ADP or AMP –inhibited by citrate

20. Regulation of the Citric Acid Cycle Isocitrate dehydrogenaseIsocitrate dehydrogenase –responds to negative feedback from NADH and H+ and ATP –is activated by ADP and NAD+

21. Regulation of Acetyl-CoA Entering the Citric Acid CycleEntering the Citric Acid Cycle –Citrate synthase (1) is inhibited by ATP or NADH Use in Fatty Acid SynthesisUse in Fatty Acid Synthesis –Fatty Acid synthase (2) is stimulated by Citrate (1) (2)

22. Applying Your Knowledge Which change in enzyme activity is observed for Citrate Synthase when levels of NADH increase?Citrate Synthase when levels of NADH increase? Isocitrate Dehydrogenase when levels of ADP increase?Isocitrate Dehydrogenase when levels of ADP increase? Fatty Acid Synthase when levels of citrate increase?Fatty Acid Synthase when levels of citrate increase? Phosphofructokinase when levels of citrate increase?Phosphofructokinase when levels of citrate increase? Thumbs Up: Enzyme Activity Increases Thumbs Down: Enzyme Activity Decreases