1. Welcome to Microbiology!

2. Why Study Microbiology? When will I EVER use this again in my life Mrs. Z?

3. EBOLA

6. CDC Chronology / Ebola http://www.cdc.gov/vhf/ebola/outbreaks/ history/chronology.html http://www.cdc.gov/vhf/ebola/outbreaks/ history/chronology.html http://www.cdc.gov/vhf/ebola/outbreaks/ history/chronology.html http://www.cdc.gov/vhf/ebola/outbreaks/ history/chronology.html

8. December 31, 2014 CDC announced: USA Epidemic Levels

11. Meningitis Outbreak – 9/2012

12. AS OF NOVEMBER 26, 2012

13. The Swine Flu Virus

14. Chipotle Faces Another Foodborne Illness Outbreak, This Time In Boston

15. Do I have your attention yet? …

16. Not yet? … Have you heard of MRSA?

17. OK – enough… OK – enough… On to some paper work!

18. Classroom Rules Micro / Forensics / Zoo Have a positive attitude! Have a positive attitude!

19. Respect Each Other

20. Respect this Room & Building NOTIFY US IMMEDIATELY - If there are any marks on the desk – PLEASE NOTIFY US IMMEDIATELY - If there are any marks on the desk – PLEASE If you don’t – you may get blamed for it by the next student, in the next class. If you don’t – you may get blamed for it by the next student, in the next class. If there is an accident in lab, class or damage to anything in this room – LET ME KNOW – “Life Happens” – it’s OK! If there is an accident in lab, class or damage to anything in this room – LET ME KNOW – “Life Happens” – it’s OK!

21. BE ON TIME! GET IN YOUR SEAT BEFORE THE BELL RINGS! – Especially 1 st Block! Get right to work! No Sleeping in Class

22. Raise your hand when you have a question THINK / QUESTION / INQUIRE & DOUBT - Ask questions! : Remember: There is no such thing as a dumb question.

23. Be Prepared for Class

24. Things can get confusing – Never hesitate to ask…

25. NO EATING OR DRINKING IN CLASSROOM AT ANY TIME

26. NO CELL PHONES! NO CELL PHONES! “ Each of you have an assigned pocket “ Each of you have an assigned pocket

27. Dispose of waste properly This is NOT PROPER!

28. Do not get up in the middle of class to throw something away or sharpen your pencil

29. SAFETY IS #1

30. Lab Safety Safety equipment; procedures… FIRE DRILLS / Code RED Safety equipment; procedures… FIRE DRILLS / Code RED

31. Know What all Signs & Symbols mean

33. Cleanliness is Safety WASH HANDS EVERYDAY BEFORE YOU LEAVE THE CLASSROOM WASH LAB TABLE WITH BLEACH BEFORE AND AFTER USE

35. Follow the Kenston Handbook Rules

37. Microbiology Micro = Small / Biology = “Study of Life” Micro = Small / Biology = “Study of Life” Microorganisms = too small to be seen without a microscope Microorganisms = too small to be seen without a microscope Prokaryotic Cells = No nucleus Prokaryotic Cells = No nucleus Eukaryotic Cells = Have a true nucleus Eukaryotic Cells = Have a true nucleus

39. Examples of Microorganisms Kingdom Archaebacteria: the most ancient of all living things. Kingdom Archaebacteria: the most ancient of all living things. Three types: Three types: thermoacidophiles thermoacidophiles methanogens methanogens Halophiles Halophiles

40. Kingdom Eubacteria The "true bacteria" they are classified by shape and Gram Stain. The "true bacteria" they are classified by shape and Gram Stain. Shapes: Shapes: Coccus = Spherical Coccus = Spherical Bacillus = Rod Shaped Bacillus = Rod Shaped Spirillum = Spiral Spirillum = Spiral

41. Bacteria usually smaller than a Eukaryotic Cell Skin Cell

43. Bacterial Endospore

44. Bacterial Cell

46. Coccus or Round (sphere shaped)

47. Bacillus

48. The Spirals

49. There are a few other Kingdoms we will consider …

50. Kingdom: Fungi Eukaryotic - Classified by the type of spores they use to reproduce with. Eukaryotic - Classified by the type of spores they use to reproduce with.

51. Some Fungi are “microscopic” Athlete’s Foot; Ringworm and Penicillin (for example)

52. Kingdom Protista Eukaryotic – Classified by how they move (flagellum, cilia or pseudopodia) Eukaryotic – Classified by how they move (flagellum, cilia or pseudopodia)

53. Euglena (flagellum)

54. Paramecium (cilia)

55. Amoeba (pseudopodia)

56. Many Protists Cause Disease Malaria Malaria African Sleeping Sickness African Sleeping Sickness Giardia Giardia Toxoplasmosis Toxoplasmosis Often transmitted by a “vector” (insect for example) Often transmitted by a “vector” (insect for example)

58. Viruses = “poison” Not in a Kingdom … Not in a Kingdom … Why not? Why not?

60. Viruses They are parasitic They are parasitic They need a host to reproduce They need a host to reproduce (the virus hijacks the cells organelles – and makes copies of itself) (the virus hijacks the cells organelles – and makes copies of itself) They have DNA or RNA (not both) They have DNA or RNA (not both)

61. AGAR AGAR

62. AGAR (ā'gär) n. (ā'gär) n. Made from algae (seaweed – from the ocean). Made from algae (seaweed – from the ocean). And as a stabilizer and thickener in many food products. And as a stabilizer and thickener in many food products. Home for bacteria Home for bacteria The bacteria do not EAT agar; it is a lattice work for them to hang on The bacteria do not EAT agar; it is a lattice work for them to hang on

65. Petri Dish (Glass or Plastic) – a container that we will pour the agar into. (Glass or Plastic) – a container that we will pour the agar into.

66. Terms: Sterile = “clean” – no life Sterile = “clean” – no life Contaminated = “dirty” not sterile un-pure Contaminated = “dirty” not sterile un-pure Inoculate = introduce an organism into another ( on agar in Petri dish for example ) Inoculate = introduce an organism into another ( on agar in Petri dish for example ) Pathogenic = Causes disease Pathogenic = Causes disease Autoclave = “Sterilizer or pressure cooker”, equipment and agar put into canister for at least 20 minutes at 250 degrees. Autoclave = “Sterilizer or pressure cooker”, equipment and agar put into canister for at least 20 minutes at 250 degrees.

68. Coccus or Round (sphere shaped)

69. Bacillus

70. The Spirals

72. Bacterial Colonies on Petri Dishes

73. Sample Petri Dish Results

74. Agar contains all of the following: Beef Extract = Protein Beef Extract = Protein Yeast Extract = Growth Factors, Yeast Extract = Growth Factors, Sucrose or Dextrose = Energy Sucrose or Dextrose = Energy Different types of agar will grow different types of bacteria Different types of agar will grow different types of bacteria

76. Gram Stain

77. Antibiotic Testing