1. The effect of hot springs components Natsumi Kato, Yuka Hirano

2. http://nv-med.mtpro.jp ' There are many hot springs in Japan!! High temperature groundwater in Japan

3. “Awara hot spring” in Fukui! http://www.city.awara.lg.jp/

4. Hot spring qualities and effects Spring qualitySpring effects Carbonated springHypertension, arteriosclerosis Chloride springMuscle and arthralgia pain Hydrogen carbonated spring Gout, diabetes Saline sulfur springHypertension, diabetes Sulfur springHypertension, arteriosclerosis Radioactive spring Hypertension, gout, arteriosclerosis From “Onsenkyo spring quality and efficacy” http://www.onsenkyo.com http://www.onsenkyo.com

5. Hypothesis Carbon dioxide gas has blood circulation promoting effects The blood is flowing from the heart → Lively movement of the heart causes lively blood circulation If we give carbonated water to the heart, motion of the heart becomes more active

6. Even if we use human beings, we can’t observe objectively. Is there a creature we can use as a model creature? whose heart structure is almost the same as human beings Quail Quail Coturnix japonica

7. ① The way of adjusting the quail embryo A fertilized egg kept at 37 ℃ for 72 hours Make a tiny hole in the tip of the quail egg

9. ② Adding carbonated water  Model of carbonated spring use “IROHASU sparkling” water (produced by Coca-Cola Company in Japan)  Directly add about 15 mL of this water  Use a solution that was heated and had carbon dioxide removed for the control experiment http://www.cocacola.co.jp/

10. ③ Observation

11. Result  Observation of each example. Heart rate (carbon dioxide in) Heart rate （ except carbon dioxide ） Before solution add ０．６ times/second ０．３ t/s 2 minutes after adding liquid ０．５ t/s ０．３ t/s 2~4 minutes after adding liquid ０．４ t/s ０．３ t/s

12. consideration Can not think carbon dioxide influences the quail’s heart rate. When adding carbonated liquid into quails, looked for a big change in heart rate.

13. ＋ CO ２ － CO ２－ CO ２・４ min ＋ CO ２・４ min

14. Future challenge  Increase examples of quails  Show heart rate objectively  Establish situations of taking a bath in a hot spring  Examine various effects according to spring qualities

15. Thank you for listening!!!