1. 2011 Status of DED Tolerant American Elm Linda Haugen, Plant Pathologist USDA Forest Service, Forest Health Protection St. Paul, Minnesota

2. Overview of what you should know from 2009 talk… 1.DED tolerant American elm cultivars, their characteristics and availability. 2.Research at the Univ of MN– especially pruning work, and St. Croix elm. 3.Status of research at Delaware, Ohio, Forest Service NRS location (Slavicek).

3. 2011 Status of American Elm What’s new. We need more elms. Elm “Restoration” Demo Sites. DED Tolerance Enriched Seed.

4. Dr. Kathleen Knight, Northern Research Station. Lots of Talk… Strategies and approaches. Restoration ecology. Modeled vegetation response to EAB infestation, particularly invasives. Current work in Ohio, may expand as funded. What’s new

5. We need more elms Princeton Highly available * Valley Forge (Amer. 3) Moderately to highly available. * New Harmony (Amer. 680) Low to moderately available. * Lewis & Clark ‘Prairie Expedition’ TM Limited availability Jefferson (N3487) Beginning availability * American Liberty (W502, W503, W505, W507, W510, and M-8) Only through the Elm Research Institute (ERI). Independence (W510) Yes, as part of American Liberty Delaware 2 (Delaware) Not commercial * R18-2 Not commercial * St. Croix Not yet. MN selection Commercial Availability NRS

6. We need more elms Finding more DED tolerant American elms Long-term projects: > 8 years to screen Chippewa NF project: Started 2009 Cold tolerance x DED tolerance Nature Conservancy project to start in New York/New England? “Lessor elms”: IN, IL, OH, MI We need more, from rest of range New database– web entry

7. We need more elms

9. Elm Restoration Sites Elm Restoration Demonstration in the Upper Mississippi 2 upland sites, 2 bottomland “Wild land” site “Free to Grow” Planted 2005 and 2007 INTENSE protection 30 to 40 trees per site Princeton Valley Forge Delaware VF x New Harmony VF x R18-2 VF x Princeton VF x Delaware

10. Elm Restoration Sites

11. Other NRS “restoration sites” Original sites in Ohio. (5 or 6) Partnered with Nature Conservatory (Christian Marks) in New England to plant 30 sites.

12. DED Tolerance Enriched Seed Heritability of DED Tolerance Eshita, Slavicek and Kamalay. 2003. Generation of American Elm Trees with Enhanced Tolerance/Resistance to Dutch Elm Disease through Genetics. R18-2 x Valley Forge New Harmony x Delaware 2 New Harmony x R18-2 New Harmony x Valley Forge Planted in 1995, inoculated w/ DED in 2002. Evaluated after 4 weeks and 8-9 weeks. Several progeny showed few symptoms even at 8-9 weeks: 38/321 at 5% or less 73/321 at 10% or less “Enriched Seed”= seed from crosses of DED tolerant American elm parents Smalley and Guries. 1993. Breeding Elms for Resistance to Dutch Elm Disease. In F2 elm population, their best “families” yielded up to 80% DED resistant individuals.

13. DED Tolerance Enriched Seed Field use of DED tolerance enriched seed: NRS has “Seed Orchard” Potentially use to propagate seedlings for field trials of restoration plantings. Perhaps 2012? “Public lands” partners? State Nurseries?

14. Heterobasidion Root Disease (HRD) NA-2009-02. Detection Tools, Risk Analysis and Management Guidelines for Annosum Root Rot in the Northeastern Area US. JJuzwik, DLindner, WI DNR, GStanosz, SPFO Objectives: DNA-based sampling protocol. Science-based risk analysis system and associated management guidelines. BRIEF DESCRIPTION OF THE PROJECT: Develop and test sampling methodology for HRD. Collect data on risks of introduction and risks of intensification, and potential impact. -- inoculum availability (i.e. viable fruiting bodies) -- time of basidiospore dispersal throughout the year -- Importance of edaphic, prior land use, and stand variables Document H. annosum occurrence within each State (baseline regional database). Finalize risk analysis system and management guidelines for HRD in Northeast.

15. Heterobasidion Root Disease (HRD)

16. In the Field: Cordless drill used to collect wood sample into a micro-centrifuge tube. Materials packed into a “kit”. In the lab: Full profile of fungal DNA in sample. J. Juzwik has been learning technique. Many samples processed synchronously = cost efficiency. Only received 40 samples last year! Heterobasidion Root Disease (HRD)

17. Expand sampling– perhaps send out NRS or NA samplers. Include “mystery root disease pockets”, not just HRD suspects. Heterobasidion Root Disease (HRD) You can help! You can BE HELPED! Contact J. Juzwik or L. Haugen to participate!