1. Nanoparticle-Based Bio-Bar Codes
Angela Wu
October 12th, 2008

3. Background Target Amplification for DNA (PCR) Protein Targets?
Interest for research, forensic and clinical applications
Protein Targets?
Improve medical diagnostics and proteomics

4. Immuno-PCR Tag protein targets with oligonucleotide markers
Amplify with PCR
Use DNA detection to identify target

5. Downfalls of Current Technology
Low ratio of DNA sequence to Ab
Slow target-binding kinetics
Complex conjugation chemistries involved in linking DNA to Ab
PCR requirements

6. Bio-Bar Code Assay Magnetic microparticle probes with Ab
Nanoparticle probes that are encoded with DNA and Ab that can sandwich the target
Magnetic separation
Dehybridization of the oligonucleotides
Determination of presence of target protein by identifying sequence

7. Bio-Bar Code Assay

8. Advantages Target does not need to be immobilized
Faster binding kinetics
High ratio of PCR-amplifiable DNA to labeling Ab
Increased sensitivity
No complicated conjugation chemistry
No additional Ab or DNA-protein conjugates

9. Significance Ultrasensitive
Proteins can be detected at 30 attomolar concentrations
With PCR, at 3 attomolar
Prostate-specific antigen (PSA) detection for prostate cancer and breast cancer

10. Questions