1. Malaria and Dengue A mosquito-based approach

3. Design anti-pathogen genes Mosquito Population

4. Culex quinquefasciatus West Nile fever

5. An. darlingiAn. aquasalis Ae. aegypti Malaria

6. Ae. aegypti Ae. albopictus Dengue fever

8. Recombinant antibodies (scFv - single chain antibody) Anti-pathogen genes Active peptides Protein domains

9. 1A10-2 1B7 – E protein 3H5 9A3D-8 2H2 – prM Recombinant antibodies (scFv - single chain antibody)

11. 1A10-2-scFv Sindbis expression system VH VL his L VH VL L

12. RArmLArm VG-Promoter 3xP3 EGFP SV40 5’UTR cDNA 3’UTR pBac[3xP3-EGFP, Vg-effector]

13. 14,50016 14,50015 14,00014 10,50013 10,00012 7,50011 6,00010 4,8009 618 417 406 345 314 103 52 01 Sporozoites/glandscFv-Transgenic 21 99.8% Plasmodium gallinaceum and Aedes aegypti

14. Promoter Effectors Molecules 5’UTR 3’UTR Transgene

15. Aedes aegypti fat body transcriptome 50.82% 7.97% 5.16% 30.05%

16. 130 transcripts ♂ ♀ Macro array

17. Proteomic approach on hemolymph proteins ♀ Sucrose 24 hs 48 hs 72 hs OV BF

18. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20

19. Non-infected P.gal infected Pg1

20. Mosquitoes Population

21. The haplotype network for mitochondrial ND4 from Aedes aegypti populations from Brazil. Each line in the network represents a single mutational change. White circle represents a theoretical interior node. 1 297 22 20 1517 18 13 14 23 Senegal Uganda, Singapore, Cambodia and Tahiti 5 3 José Eduardo Bracco e Dr. Maria Anice Sallun

24. Ae. albopictus Ae. aegypti Specie-specific markers

25. Dr. Margareth L. Capurro ICB – USP Bianca Burini Fernanda Rosa – MT - FAPESP Ricardo V. Araújo – DD - CNPq Fabiana M. Feitosa – DD - FAPESP JoãoV. Camargo e Silva – DD - CAPES Daniela G. Rodrigues – MT - CNPq Ceres M. de Miranda – MT - CNPq Márcia Sperança – PosDoc - FAPEMA José Eduardo Bracco – DT- (SUCEN) Alexandre Moura – technical support Dr. José Eduardo Levi IMT - USP Dr. Anthony A. James UCI Dr. Ken Olson Colorado State Dr. Maria Anice Sallun FSP - USP Supported by FAPESP Dr. Tania Bijovsky ICB - USP