1. Learning the Basics The tutorial in this section is designed to introduce you to the basic image acquisition and analysis tools within QED. You can go through it in simulated mode or you can configure your camera when you go through the tutorial. In the Advanced Tutorial we will cover the configuration of your automated microscope The Tutorial is structured as follows: In the area between the 2 red bars a small description is given for the current task. In the main area you will see descriptions of the tools and windows. You will also see descriptions of individual tasks you can do, they will be numbered in sequence. The tutorial will cover features available in QED Capture, QED Image and QED In Vivo. Not all features are available for all three packages. Learning the Basics

2. Color Imaging Modes Tool Learning the Basics The QED Color Panel allows you to do easy multi-color acquisition. It can automatically acquire up to six arbitrary channels. It also support flexible color choice. Each channel has independent contrast, brightness and gamma settings. They can be adjusted interactively or automatically with on-the-fly results in the color composite. 1) Select the Color Acquire Panel. 2) Click the Auto Expose for all channels. 3) Change the channel viewing modes; on, off or blinking. 6) Perform linear Image Enhancement through the sliders. 8) Acquire all channels with the new settings for the individual channels. 7) Perform precise image registration with memory

3. Using the Stored Configurations for acquisition. 1) Click on the MIC buttons to see the associated Microscope configuration for each Image Channel. Every Microscope Configuration can be assigned to an Imaging Mode. An Imaging Mode is a Acquisition with pre-defined parameters like: exposure time, binning, gain etc. The Imaging Modes Tool uses the first three Microscope Configuration for the first three colors. You can change the order or you can select your new Microscope Configuration. 1) Click the Acquire all button to acquire the Color Image (in this case only FITC and Cy3 are checked for Acquired). 3) Select your new Microscope Configuration in the Configuration Drop-down button.

4. Saving an Image 2) To save the image file, select the folder in which you want to place it, specify a name for the file, and click on the Save button. 1) To save an image to disk, you must click on the Save button. When you click on the Save button, a custom file specification dialog box appears as shown here. You are only able to save the image as a BMP file (Windows) or a PICT file (Macintosh). 3) You can fill the Image Information in the text entry fields in the top section of this window in any way you please. We suggest that you specify standards for filling these fields. 4) You can elect to apply the Brightness, Contrast, and Gamma values that were set in the Acquisition control panel to the pixel values in the saved image file. If you activate this check box, the image you get in your host program (Adobe PhotoShop, NIH- Image, etc.) will look like the image in the imaging area at the time you acquired the image. 5) The Annotation and Scale Bar tools in the main toolbar let you superimpose text and/or a micron calibration bar directly onto your image. The Paste Annotation check box in the Save Image window determines whether or not annotations added with these tools overwrite pixel values in the saved image. Finally we will cover how to save an individual single or multi color images image. Time Lapse sequences save automatically.