1. Prokaryotes (Archaea and Bacteria) Chapter 28

2. 2 The First Cells Microfossils are fossilized forms of microscopic life -Oldest are 3.5 billion years old -Isotopic analysis and concentration of carbon-12 in fossils suggests that carbon fixation was active as much as 3.8 BYA

3. 3 The Cyanobacteria Stromatolites are mats of cyanobacterial cells that trap mineral deposits -Oldest are 2.7 billion years old -Lipids were found in ancient rocks

4. 4

5. 5 Flagella, spin like propellers Composed of the protein flagellin

6. 6 Prokaryotic Features Metabolic diversity -Two types of photosynthesis -Oxygenic = Produces oxygen -Anoxygenic = Nonoxygen producing - E.g: Sulfur and sulfate -Chemolithotrophic prokaryotes derive energy from inorganic molecules

7. 7 Bacteria vs. Archaea Plasma membrane -Bacterial-Connected to glycerol by ester linkages -Archaeal-Connected to glycerol by ether linkages

8. 8 Most prokaryotes have one of 3 basic shapes -Bacillus = Rod-shaped -Coccus = Spherical -Spirillum = Helical-shaped Prokaryotic Shapes

9. 9 Maintains shape and protects the cell from swelling and rupturing Consists of peptidoglycan -Polysaccharides cross-linked with peptides Cell wall is the basis of the Gram stain The Bacterial Cell Wall

10. 10 Two main types -Gram-positive bacteria -Thick peptidoglycan -Teichoic and lipoteichoic acids -Gram-negative bacteria -Thin peptidoglycan -Have an outer membrane -Contains lipopolysaccharide The Bacterial Cell Wall

11. 11 The Bacterial Cell Wall

12. 12 The Bacterial Cell Wall

13. 13 Internal Structure Internal membranes -Invaginated cell membrane -For respiration or photosynthesis Endospores -Highly-resistant structures -Released upon cell lysis -Can germinate back to normal cell

14. 14 Prokaryotic Genetics Prokaryotes do not reproduce sexually However, they undergo horizontal gene transfer, which is of three types -Conjugation = Cell-to-cell contact -Transduction = By bacteriophages -Transformation = From the environment

15. 15 Conjugation Transfer of the F plasmid occurs through the conjugation bridge The end result is two F + cells -R (antibiotic resistance) plasmids -Virulence plasmids (E. coli O157:H7 strain)

16. 16 Transduction Viruses package bacterial DNA and transfer it in a subsequent infection

17. 17 Transformation Natural transformation -DNA that is released from a dead cell is picked up by another live cell...Horizontal gene transfer (no cell fusion so not sexual !!)

18. 18 Prokaryotic Metabolism Acquisition of Carbon -Autotrophs = From inorganic CO 2 -Heterotrophs = From organic molecules Acquisition of Energy -Chemolithotrophs = From inorganic chemicals -Phototrophs = From sunlight

19. 19 Prokaryotic Metabolism Photoautotrophs -Cyanobacteria Chemolithoautotrophs -Nitrifiers Photoheterotrophs -Purple and green nonsulfur bacteria Chemoheterotrophs -Majority of prokaryotes -Use organic molecules for C and energy

20. 20 Prokaryotic Metabolism Type III secretion system -Found in many Gram-negative bacteria -Used to transfer virulence proteins directly into host cells -Yersinia pestis – Bubonic plague -Pseudomonads – Plant pathogens -Blights, soft rot, wilts

21. 21 Human Bacterial Disease Tuberculosis -Mycobacterium tuberculosis -Afflicts the respiratory system -Mutidrug-resistant (MDR) strains are very alarming

22. 22 Human Bacterial Disease Dental caries (tooth decay) -Plaque consists of bacterial biofilms -Streptococcus ferments sugar to lactic acid -Tooth enamel degenerates Peptic ulcers -Helicobacter pylori is the main cause -Treated with antibiotics

23. 23 Gonorrhea -Neisseria gonorrhoeae -Can pass from mom to baby via birth canal -Can cause pelvic inflammatory disease (PID) Chlamydia -Chlamydia trachomatis -Can cause PID and heart disease Sexually transmitted diseases (STDs)

24. 24 Beneficial Prokaryotes Prokaryotes are crucial to chemical cycles -Decomposers release a dead organism’s atoms to the environment -Photosynthesizers fix carbon into sugars -Nitrogen fixers reduce N 2 to NH 3 (ammonia)

25. 25 Beneficial Prokaryotes Bacteria are used for bioremediation -Remove pollutants from water, air and soil -Exxon Valdez oil spill