1. SELDI-TOF results Pigs: Streptozotocin induced diabetic and control pigs Both fed a high unsaturated fat Mediterranean diet or A high saturated fat Cafeteria diet: 4 groups in each analysis Proteomics: SELDI-TOF arrays: CM10, pH7 buffer CM10, pH 4 buffer H50 IMAC30 Sample enrichment: Proteominer 4 fractions (fr)

2. CM10 pH7, fr1 M/ZP-value 47840.000896 62490.001759 45740.005584 49950.007090 26540.007717 46220.008207 222590.009799

3. M/Z 4784, CM10 pH7, fr1

4. M/Z 6249, CM10 pH7, fr1

5. M/Z 4995, CM10 pH7, fr1

6. CM10 pH7, fr2 M/ZP-value 62480.001131 26540.002628 47840.002959 124700.004171 68030.005150 36470.005923 64320.007451 73960.007603 49990.007648 131140.009235 896560.009593

7. M/Z 6248, CM10 pH7, fr2

8. M/Z 3647, CM10 pH7, fr2

9. M/Z 6432, CM10 pH7, fr2

10. M/Z 13114, CM10 pH7, fr2

11. CM10 pH7, fr3 M/ZP-valueM/ZP-value 285370.00025985460.001510 142640.000333231880.002122 73910.0003901186520.002320 49970.00048539070.002462 118100.000517180140.002767 897450.00057283360.002782 69590.000711671280.003214 137850.00087064190.003367 212610.001100276580.003713 103220.0011451233540.004163 562770.001190360590.005209 66230.00119136500.005701 415010.00122278280.007046 86510.001366832610.008554 85020.001455

12. M/Z 28537, CM10 pH7, fr3

13. M/Z 11810, CM10 pH7, fr3

14. M/Z 89745, CM10 pH7, fr3

15. M/Z 6959, CM10 pH7, fr3

16. M/Z 10322, CM10 pH7, fr3

17. M/Z 6623, CM10 pH7, fr3

18. CM10 pH7, fr4 M/ZP-value 118410.000477 64270.001857 66210.002160 347920.002490 102420.002639 65040.002874 45780.005327 49950.006458 39070.007978

19. M/Z 11841, CM10 pH7, fr4

20. M/Z 6427, CM10 pH7, fr4

21. Principal component analysis CM10 High Stringent fr3

23. CM10 pH4(Low Stringent), fr1 M/ZP-value 83330.000857 223400.001196 107060.001219 85450.001844 21220.003138 76160.004524 68030.004710 98870.004771 49880.008042 62080.008068 126380.009241

24. M/Z 8333, CM10 pH4, fr1

25. M/Z 22340, CM10 pH4, fr1

26. M/Z 6803, CM10 pH4, fr1

27. CM10 pH4(Low Stringent), fr2 M/ZP-value 58610.00070571680.003184 131030.000855287800.004085 68010.00128769580.004511 39150.00141142720.005260 64310.00141228530.005565 86480.00142978280.006583 85480.001550347730.006602 897040.00220662070.006893 64880.00244088340.006976 50000.00258073970.009650

28. M/Z 5861, CM10 pH4, fr2

29. M/Z 6431, CM10 pH4, fr2

30. M/Z 8648, CM10 pH4, fr2

31. M/Z 28780, CM10 pH4, fr2

32. CM10 pH4, fr3 M/ZP-value 84990.000379138250.002813 176800.000572166760.002898 347540.000688276560.003163 86500.000852561890.003296 85390.00086083910.003330 85500.001347415180.003538 108850.00145569590.004262 285270.001493837750.004428 144410.00154439440.004991 73900.001709231820.005046 49960.001785951610.005775 83360.002360554750.006396 41620.002700

33. M/Z 8499, CM10 pH4, fr3

34. M/Z 34754, CM10 pH4, fr3

35. M/Z 10885, CM10 pH4, fr3

36. M/Z 28527, CM10 pH4, fr3

37. CM10 pH4, fr4 M/ZP-value 285000.000499 49950.000553 362250.000782 82100.000797 83310.000842 347420.001030 37260.001134 144370.001154 86470.001545 85530.001857 39210.002626 69560.003853

38. M/Z 28500, CM10 pH4, fr4

39. M/Z 4995, CM10 pH4, fr4

40. M/Z 8210, CM10 pH4, fr4

41. M/Z 8553, CM10 pH4, fr4

42. H50 arrays, fr1 M/ZP-value 108740.00076441710.004718 83420.001264131050.004861 85490.00208650000.00507 69520.002128537240.005154 85340.002256145530.005617 86410.00254542680.005744 69670.00260854570.006031 83280.00269988350.006367 71670.003131147100.006705 435040.003549953570.008911 554680.004025285040.009478

43. M/Z 10874, H50, fr1

44. M/Z 8342, H50, fr1

45. M/Z 6952, H50, fr1

46. M/Z 53724, H50, fr1

47. M/Z 14553, H50, fr1

48. M/Z 5457, H50, fr1

49. H50, fr1

51. H50 arrays, fr2 M/ZP-valueM/ZP-value 86420.00093185520.002610 285130.001352212250.004057 73920.001389142600.004135 85350.00139083430.004922 39040.001460414660.005339 83290.0016071260550.006718 145470.001696991120.008519 41600.00211668000.008870 208330.002256

52. M/Z 8642, H50, fr2

53. M/Z 28513, H50, fr2

54. M/Z 7392, H50, fr2

55. M/Z 14547, H50, fr2

56. M/Z 20833, H50, fr2

57. H50 arrays, fr3 M/ZP-valueM/ZP-value 71620.000593142560.003739 69560.001218285110.004824 341930.00138485520.005648 82010.00230986470.006326 166710.00268341600.008796 120020.003096

58. M/Z 7162, H50, fr3

59. M/Z 16671, H50, fr3

60. M/Z 28511, H50, fr3

61. H50 arrays, fr4 M/ZP-value 120070.000516 81930.000606 142520.000729 82090.001678 1640830.006688 281760.009644

62. M/Z 12007, H50, fr4

63. M/Z 8193, H50, fr4

64. M/Z 14252, H50, fr4

65. M/Z 8209, H50, fr4

66. M/Z 28176, H50, fr4

67. IMAC arrays, fr1 M/ZP-value 21180.001235 223660.001305 48990.004580 49140.005734 201330.006144 256020.006249

68. M/Z 2118, IMAC, fr1

69. M/Z 4899, IMAC, fr1

70. M/Z 25602, IMAC, fr1

71. IMAC arrays, fr2 M/ZP-valueM/ZP-value 88350.000597102410.004374 62440.00166742700.004515 83290.002285131080.007803 201210.00245449930.008566 83430.00296645720.009452 898560.00386462050.009685 347610.004239

72. M/Z 8835, IMAC, fr2

73. M/Z 6244, IMAC, fr2

74. M/Z 34761, IMAC, fr2

75. M/Z 10241, IMAC, fr2

76. M/Z 13108, IMAC, fr2

77. M/Z 4993, IMAC, fr2

78. IMAC arrays, fr3 M/ZP-valueM/ZP-value 347330.00057185360.002478 66180.00059583290.002826 222310.00062539050.002888 49940.000644285140.003113 82070.000844108770.003245 102330.000913897730.003266 73860.001186144320.004008 201110.00177664220.004730 52230.00222569530.007260

79. M/Z 34733, IMAC, fr3

80. M/Z 6618, IMAC, fr3

81. M/Z 8207, IMAC, fr3

82. M/Z 28514, IMAC, fr3

83. IMAC arrays, fr4 M/ZP-valueM/ZP-value 66170.00062881990.002932 102330.00066839120.003517 101540.00084192090.004326 49950.00096540400.004769 895610.001311915130.005585 142520.00187837240.008563 671560.002234336110.008898

84. M/Z 6617, IMAC, fr4

85. M/Z 8199, IMAC, fr4

86. M/Z 3912, IMAC, fr4

87. M/Z 9208, IMAC, fr4

88. M/Z 3724, IMAC, fr4