1. INCDTIM Cluj-Napoca ATOM-N 2014 21 - 24 august 2014, Constanta, Romania Nanomaterials based on Gold/Silver Nanoparticles- Kuromarin Chloride and their Applications in Medicine Liliana Olenic 1, Ioana Chiorean 2, Maria Crisan 3, Adriana Vulcu 1, Camelia Grosan 1, Lucian-Barbu Tudoran 4, Irina Kacso 1, Lidia Magerusan 1, Ioan Bratu 1 1) National Institute for Research and Development of Isotopic and Molecular Technologies, 65-103 Donath Street RO 400293, Cluj-Napoca, Romania; 2)Faculty of Mathematics and Informatics, Babeş-Bolyai –University, Cluj-Napoca, Romania, 3) Histology Department, Iuliu Haţieganu University of Medicine and Pharmacy Cluj-Napoca, Romania, 4) Babeş-Bolyai University, Electron Microscopy Centre, Romania, Structural Biology and Cell Biology Histology Department, Cluj-Napoca, Romania The antocyanins obtained from plants are organic colorants studied for applications in Medicine. In another paper [1] the effect of the natural extract made from the European Cranberrybush (Viburnum opulus L) fruits, found in Romania has been studied and its benefits on skin diseases have been emphasized. Due to the fact that in these natural extracts there exists also some other organic compounds, in the present paper, as a novelty, we focused only on the effects of the pure antocyanin., i.e. kuromarin chloride, which is the major antocyanin found in these fruits. And so, new metallic nanomaterials have been obtained. In order to get a good comparison, the same preparation method of gold/silver nanoparticles as in [1] has been used. Acknowledgement This work was elaborated in the frame project no 147/2012, through the program “Partenerships in priority areas-PN II”, developed with the support of UEFISCDI, Romania. Conclusion Gold/silver nanoparticles functionalized with pure anthocyanin (kuromarin chloride), AuNPs-Kuromarin and AgNPs- Kuromarin were prepared and characterized by UV-Vis and FTIR spectroscopy, by Transmission Electron Microscopy (TEM) and RDX. There were assessed cytotoxicity tests of the extract alone and of the functionalized nanoparticles. To study the antiinflammatory effect, keratinocytes treated with non-toxic doses of anthocyanins and nanoparticles were exposed to increasing doses of UVB irradiation. The histograms of the skin thickness for 7 patients presented an involution of diseases after treatment, fact that indicates the very good antiinflammatory effect of our nanomaterials. Nanomaterials characterization Reference: [1] M. Crisan, L. David, B. Moldovan, A. Vulcu, S. Dreve, M. Perde-Schrepler, C. Tatomir, A. G. Filip, P. Bolfa, M. Achim, I. Chiorean, I. Kacso and L. Olenic, "New nanomaterials for the Improvement of psoriatic lesions”lesionlesions", J.Mater.Chem.B, 2013,1,3152-3158. Fig 5. Psoriatic lesions; The histogram of skin Thickness for 6 patients before and after treatment with nanomaterials Fig. 1. AuNPs-kuromarin: a – TEM; b – histogram; c – UV-Vis spectra Fig. 2. AgNPs-kuromarin: a – TEM; b – histogram; c – UV-Vis spectra bc a Fig. 3. FT-IR spectra of: a – kuromarine chloride; b – AuNPs-kuromarin; c – AgNPs-kuromarin Fig. 4. RDX of: a – AuNPs-kuromarin; b – AgNPs- kuromarin b a a b c a bc Abstract