Presentation is loading. Please wait.

Presentation is loading. Please wait.

Gel Electrophoresis gel electrophoresis – moving DNA through a gel medium using an electric current Why can we move DNA with electricity? DNA has a negative.

Published byAmber Garrison Modified over 9 years ago

Similar presentations

Presentation on theme: "Gel Electrophoresis gel electrophoresis – moving DNA through a gel medium using an electric current Why can we move DNA with electricity? DNA has a negative."— Presentation transcript:

1

2 Gel Electrophoresis gel electrophoresis – moving DNA through a gel medium using an electric current Why can we move DNA with electricity? DNA has a negative charge.

3 negative electrode positive electrode agarose gel

4 Gel Medium The gel medium can be made from: 1. agarose - seaweed extract 2. polyacrylamide - artificial polymer Liquid solutions of either medium are poured into a mould and allowed to set and solidify.

5 Gel Medium The semi-solid provides resistance for DNA movement. Short DNA moves through agarose easily travels further Long DNA requires more effort to move through agarose does not move as far

6 Principles of Gel Electrophoresis http://www.youtube.com/watch?v=6_4AY3lYRgo

7 DNA Visualization DNA cannot be observed in gel coloured dyes mixed with DNA to track distance travelled stain DNA with ethidium bromide (known mutagen) UV light box to see fluorescent DNA bands

8 Ethidium Bromide

9

Download ppt "Gel Electrophoresis gel electrophoresis – moving DNA through a gel medium using an electric current Why can we move DNA with electricity? DNA has a negative."

Similar presentations

Northern blotting. Electrophoresis We can separate DNA and RNA molecules by size using agarose gel electrophoresis.

Northern blotting. Electrophoresis We can separate DNA and RNA molecules by size using agarose gel electrophoresis.
BIOTECHNOLOGY What can we do with DNA?. Biotechnology Manipulation of biological organisms or their components for research and industrial purpose Usually.

BIOTECHNOLOGY What can we do with DNA?. Biotechnology Manipulation of biological organisms or their components for research and industrial purpose Usually.
Biotechniques. Gel electrophoresis Separates molecules according to size and charge. Different sized segments of DNA cut by restriction enzymes Segments.

Biotechniques. Gel electrophoresis Separates molecules according to size and charge. Different sized segments of DNA cut by restriction enzymes Segments.
Gel Electrophoresis.

Gel Electrophoresis.
BRIDGES 2014 Agarose Gel Visualization of Restriction Enzyme Digest.

BRIDGES 2014 Agarose Gel Visualization of Restriction Enzyme Digest.
Biotech Lab #5 DNA Goes to the Races “Gel electrophoresis”

Biotech Lab #5 DNA Goes to the Races “Gel electrophoresis”
By: Grace Forster and Ashlyn Cowan.  a process that seperates large molecules (including nucleic acids or proteins) by size, electric charge, and physical.

By: Grace Forster and Ashlyn Cowan.  a process that seperates large molecules (including nucleic acids or proteins) by size, electric charge, and physical.
SEPARATION OF DNA FRAGMENTS BY LENGTH. Organic molecules such as DNA are charged. DNA is negatively charged because the phosphates (red circles) that.

SEPARATION OF DNA FRAGMENTS BY LENGTH. Organic molecules such as DNA are charged. DNA is negatively charged because the phosphates (red circles) that.
“Gel electrophoresis”. Gel electrophoresis is a procedure for separating a mixture of molecules through a stationary material (gel) in an electrical field.

“Gel electrophoresis”. Gel electrophoresis is a procedure for separating a mixture of molecules through a stationary material (gel) in an electrical field.
General Genetics.  To learn how to prepare agarose gel electrophoresis.

General Genetics.  To learn how to prepare agarose gel electrophoresis.
Lab. 3 Gel Electrophoresis

Lab. 3 Gel Electrophoresis
Prepered by:- Rana Al-Turki

Prepered by:- Rana Al-Turki
GEL ELECTROPHORESIS Lab.5. Gel electrophoresis is a method used to separate nanoparticles by charge or size. It is used to: 1. separate a mixed population.

GEL ELECTROPHORESIS Lab.5. Gel electrophoresis is a method used to separate nanoparticles by charge or size. It is used to: 1. separate a mixed population.
Gel Electrophoresis.

Gel Electrophoresis.
Marie Černá, Markéta Čimburová, Marianna Romžová

Marie Černá, Markéta Čimburová, Marianna Romžová
Agarose Gel Electrophoresis 1Dr. Nikhat Siddiqi. Agarose is a linear polymer made up of the basic repeating unit of agarobiose which comprises alternating.

Agarose Gel Electrophoresis 1Dr. Nikhat Siddiqi. Agarose is a linear polymer made up of the basic repeating unit of agarobiose which comprises alternating.
Agarose gel electrophoresis

Agarose gel electrophoresis
Agarose gel electrophoresis BCH 333 [practical]. Agarose gel electrophoresis: is a method of gel electrophoresis used in biochemistry and molecular biology.

Agarose gel electrophoresis BCH 333 [practical]. Agarose gel electrophoresis: is a method of gel electrophoresis used in biochemistry and molecular biology.
Collect Buccal Cells. PCR Polymerase Chain Reaction DNA/gene amplification.

Collect Buccal Cells. PCR Polymerase Chain Reaction DNA/gene amplification.
Gel Electrophoresis Do you want a footer?.

Gel Electrophoresis Do you want a footer?.

Similar presentations

Ads by Google