1. Haemoglobinopathies - Lab Tests Solubility:

2. Haemoglobinopathies - Lab Tests Solubility: For Hb S Cause the abnormal Hb S to precipitate. Reducing substances induce sickling in vitro. Sodium metabisulphite and sodium dithionite False Negative Result Anaemia Post Tx False Positive Result Icteric/lipaemic Sample Other sickling Hb: e.g. Hb Jamaica Plain

3. Haemoglobinopathies - Lab Tests HPLC 1 Solvent reservoirs, 2 Solvent degasser 3 Gradient valve 4 Mixing vessel 5 High-pressure pump, 6 Switching valve in "inject position", 6' Switching valve in "load position", 7 Sample injection loop, 8 Pre-column (guard column),guard column 9 Analytical column, 10 Detector 11 Data acquisition, 12 Waste

4. Haemoglobinopathies - Lab Tests HPLC Variant Hb Raised Hb A 2

5. normal Haemoglobinopathies - Lab Tests

6. sickle cell trait - AS Haemoglobinopathies - Lab Tests

7. sickle cell disease - SS Haemoglobinopathies - Lab Tests

8. Hb C carrier - AC Haemoglobinopathies - Lab Tests

9. raised Hb A 2 Haemoglobinopathies - Lab Tests

10. Electrophoresis acid pH alkali pH 2ControlNormal 3ControlAS 4ControlAC 5ControlSS 6Control  -thalassaemia trait (note similarity to 2) 7TestAS 8TestAS 9TestEE

11. Haemoglobinopathies - Lab Tests Blood Count Data: Hypochromic microcytic (relative) erythrocytosis MCH Target cells (due to membrane damage from globin chains) Sickled cells Crystallised Hb

13. Haemoglobinopathies - Lab Tests Given a Fresh Sample: Hb H preparation