1. Development of a Volatile Delivery System (Ongoing Research) Matt Bennett Dr. Mark E. Whalon Dept. of Entomology Andrea Coombs Dept. of Entomology

2. Background Robert J. Bartelt, USDA –Journal of Chemical Ecology 1990 1997

3. Objectives of Project Build Bartelt’s VDS Figure out how it works Make Improvements Research –Bioassays Attractants Repellants

4. What is it? Volatile Mixes –Chemicals –Pheromones –Other

5. Definitions Volatile Chemicals –a compound released from a plant that can either attract, repel, or otherwise influence insect behavior. Pheromones –a chemical produced by insects that illicits behaviors from other insects of the same species.

6. Why? Monetary Losses Realistic Approach Bioassays With Less Hassle FQPA Model

7. Insect Studied Plum Curculio –Conotrachelus nenuphar –Weevil –Family: Coleoptera Plants (Roseaceae) –Cherry –Hawthorn –Apple

9. Plant Studied Apple –Apple Buds –Crescent Scars –Larvae

11. How it Works Zero Chromatographic Air –gasses, hydrocarbons, moisture Hydrocarbon Trap –organic compounds Air Flow Regulators –Make up air

13. How it Works Zero Chromatographic Air –gasses, hydrocarbons, moisture Hydrocarbon Trap –organic compounds Air Flow Regulators –Make up air –Normal Flow

15. How it Works (cont’d) Chambers –Volatile –Pheromone

18. How it Works (cont’d) Splitter System –Restrictor Needles –Volatile mix

20. How it Works (cont’d) Backpressure Created

22. How it Works (cont’d) Backpressure Created Manometers

24. How it Works (cont’d) Backpressure Created Manometers Regulation Valves

26. How it Works (cont’d) GC Port –Quantitative Analysis

28. How it Works (cont’d) GC Port –Quantitative Analysis Exit to Insect Chamber –Heated –Air Pulled Through

30. Advantages Versatility Options Ratios Closer to Reality

31. Improvements (thus far) Larger Size –Easier to Work With –More Volatiles SPME Port –GC Analysis Insect Chambers –Y-chamber

32. Acknowledgements Dr. Mark E. Whalon –Department of Entomology, MSU Andrea B. Coombs –Department of Entomology, MSU Robert J. Bartelt –USDA/ARS/NCAUR Kevin W. Youngs